Whole IgG Affinity-Purified Secondary Antibodies by Jackson ImmunoResearch Laboratories Inc.

"Bright and stable fluorescence in secondaries. Antibodies with cross-reactivity removed are excellent for double or triple labeling experiments"

"It gives good results, easy to use . Good results definitely saves your money and time."

"Consistently reliable antibodies with bright fluorescent labels. Customer support was also excellent when issues did arise- friendly knowledgeable, and supplied replacement."

"High quality and reproducible results with these antibodies"

"I purchased the IgM antibody. This antibody is perfect for my purposes, used in a coating of a cell culture dish for purification of a desired cell type. Thus far, I have achieved reproducible yields in combination with the secondary antibody (purchased from Milipore-Sigma). My only complaint is the amount of antibody for the price. I use this antibody quickly as it is a large step in my cell purification. However antibodies are quite costly and this brings the cost of the cell issolation and purification protocol up dramaticallly. So far, the antibody appears to last when stored appropriately for the months that we use it over."

"High quality secondary antibodies for immunofluorescent staining"

"We used for both ELISA and Western using HRP conjugated anti mouse IgG Light chain antibody , got high quality data."

"Reproducible results, good antibody. Was one of the best deals I could find for my secondary. Good deal at high quality."

"The secondary antibodies are very easy to use and are very stable. It is easy for us to attach nanoparticles on them. They are stable and are not expensive."

"These antibodies are quite easy to use and have been largely reliable in reproducing data in our hands, even between different lot numbers."

"High quality and efficiency. Quick ship. Easy to use."

"All of the secondary antibodies I have used from Jackson ImmunoResearch have been easy to use and yield reproducible results for my immunohistochemistry experiments in perfusion fixed paraffin embedded tissues."

"Jackson ImmunoResearch antibodies are a staple of our labs' research. Indeed, we have used immunocytochemistry as a primary tool in our neuroscience research for over 20 years and have solely used Jackson's secondary antibodies because of their robust signal to noise ratio. From radial retinal sections to coronal brain sections we have explored many questions with the aid of these fluorochromes."

"Work well without a lot of background. Keep well as glycerol stocks but if having problem with a previously good primary antibody it might be time to replace your secondaries."

"We have been mainly using the 2nd antibodies from Jackson for over 10 years and highly satisfied with the products. High quality 2nd antibodies at decent prices, very reproducible results, low backgrounds and are easy to use."

"I have used Donkey fluorescent-conjugated secondary antibodies for many years from the JacksonimmunoResearch for many years. They are reliable and reproducible."

"GREAT antibodies! And very EASY TO USE. You can trust Jackson Immuno Research"

"Products are easy, affordable and very good quality"

"This product is of great use in our western blotting and immunofluorescence applications. There is good amount of reproducibility"

"Both high quality and reproducibility of results were obtained saving labor time and money. Costumer service is fast and effective."

"Are good secenday abs for immunohistochemistry. easy os use."

"The secondary antibodies from JIR are excellent. I have used the antibodies labelled with fluorescent dyes since 2005, and I am allways satisfied with the results. The grade of antibodies dedicated for multiple labelling perfom very well. Images of the confocal microscopy showing aplication of the reviewed andtibodies could be seen in our scientific papers (e.g. doi: 10.1016/j.peptides.2016.02.003; doi: 10.1007/s00441-016-2556-x.; doi: 10.1007/s00441-015-2352-z.). I appreciate the manual for reconstitution and storage that allow you to store an antibody as liquid for many years. Some of them I use more than 10 years."

"The service was excellent, antibodies arrived on time and in the best condition. We did testing and antibodies worked on expected way. Considering all - we got the quality product for reasonably price."

"All the IgG secondaries from Jackson which we have used work well: bright labeling, minimal background, specific targeting against the primary antibodies."

"High-quality results! Great antibodies! You can trust Jackson Immuno Research."

"The Immunojackson secondary antibodies are reliable, stable and very sensitive. I have been using them for decades. We obtain good results. They are also a great value , We will not change."

"In general, Jackson antibody reagents for human IgG are our go-to source for developing immunoassays for plasma measurements in preclinical animal models. Inexpensive and dependable."

"I love these antibodies. They are high-quality, reliable, and yield consistent results. I will never use non-Jackson antibodies again. They are also a great value (especially compared to Sigma-Aldrich...)."

"I have been using the Immunojackson secondary antibodies couple to horse-radish peroxidase for decades . They are reliable, stable and very sensitive. I obtain good results. The tech. team was always helpful when I had special needs like preabsorbed antibodies."

"The secondary antibody is very sensitive to detect our primary antibody. Very easy to use and good results."

"I find that these antibodies are a very good product. They are highly reproducible. Easy to use and very long storage. The lyophilized antibodies work well also after the expired date."

"I have used these secondary antibodies for years and they always work great."

"This Ab is easy to use and we can get high quality and reproducible results using it. It's very specific for the light-chain. My rating for this Ab is 5 star."

"The secondary antibody we ordered from Jackson lab is the most popular one in our lab. It works well in the regular immunohistochemistry and very easy to handle. The results are pretty consistent."

"The fluorescent labeled antibodies are reliable and economic. They are stable after long storage at -20°C as specified at their data sheet."

"We use these antibodies for most of our histology and they are the go-to antibodies for fluorescent imaging. we have used the antibodies raised in donkey and goat targeting primary antibodies from mouse, rabbit, goat and chicken. The Alexa Fluor conjugated secondary antibodies have been our preference."

"Without question, I'm happy to recommend Jackson ImmunoResearch secondary antibodies to myself and others for guaranteed ECL detection of any primary antibody of the correct species! These antibodies yield a lighthouse-bright signal out in the Ocean of Uncertainty, even when majorly diluted. Practically speaking, they are quite cheap, last for years even through poor storage practices, and come in a variety of species flavors. A+"

"The antibodies come in powder format, and it is easy to resuspend according to the manufacture's instruction. If you use according to the manufacturers instruction, you get very high quality results. I did not need to contact the manufacture for any problem. I only had to do it once and they were very helpful. The antibody sold by Jackson Immuno are not very expensive and can last for a long time."

"Results are reliable, we used to use secondary antibodies from another company before, now I just use these for the last several years and they always give me repeatable results. The product itself is good. Care needs to be taken while aliquoting it. Still a bit on the pricey side and customer service though quite helpful were not able to answer a couple of my queries. Hence the rating."

"The best secondary antibodies for staining in the market"

"It is stable, works pretty good, Customer service is also excellent and the price point for their products is very competitive. We feel good using it."

"This product serves well for a experimental needs. We have been using it for several years with good reproducibility. Its is not very expensive."

"We used immunoresearch antibodies for so many years, the products are very stable."

"The antibodies from Jackson yield reproducible results, cleaner Western blot images."

"Great antibodies, low background and high specificity"

"Very good quality Reproducible results Good value for the money"

"Always work great and the price is right so never shop around for a better deal."

"I have never had any problems with the product. It always works great and always can use the same dilution, never any differences between lot numbers. I have use their antibodies from almost 10 years. Can highly recommend them."

"Easy to use reagent with great after- sale care. Reproducible results."

"I have a good experience with Jackson secondary antibodies. They always give good results; give crisp and strong staining of antibody target with neglectable or no background. Easy to used and reconstitute. These antibodies are long lasting because I have few antibodies which i purchased in 2006 but its still work and give strong and reproducible staining. They provide the best service in term of purchasing as well as technical support. The antibodies are really good value for money. They offer huge range of secondary antibodies."

"The secondary antibodies by Jackson ImmunoResearch are highly effective. They generate reproducible results. You can trust them. This is critical when testing novel primary antibodies and have difficult tissue qualities."

"We replaced all of the previous secondaries with Jackson ImmunoResearch Laboratories Inc. antibodies. Good choice of colours/reactivities/hosts. Perform very well in multi-labeling staining due to variate of pre-adsorbed versions available. Very helpful customer service. Every time I had some problem or when I was trying to optimise my protocol I always received professional assistance and lots of good tips. All in all ...my favourite secondary antibodies. Maybe not the cheapest ...but worth every penny."

"The secondary antibodies of Jackson Immunoresearch Lab. are of great quality, showing minimal cross-reaction. I always add glycerol and store them in -20°C. They function the same from the first to the last day of usage. Ordering is really easy and you have the products the next day. I am satisfied and I will continue to use these products."

"Antibody was easy to use because I tried a range of dilutions wusing a standard protocol and the signal was present. I did not have to use any service or ask people from the company to go along. Antibody is very reliable and lasts long because of the microgramm I get for the money and because I still use it with great results although expiration date is already over."

"We used the anti-cat IgG (H+L) antibody for detection of cat antibody binding to our peptide microarrays. We labeled the antibody ourselves with DyLight680. We got very strong and clear results with the anti-cat IgG (H+L) antibody. From my experience the Jackson antibodies are very specific for their Ig subtype and species. I really like to work with them."

"We often use secondary antibodies from Jackson ImmunoResearch in our laboratory that present high effectiveness and are very easy to use."

"The secondary antibodies from Jackson are easy to handle, effective, very reproducible (even through batches) and have very low background. The ratio price/quantity is very good, as well as the costumer and post sales services."

"Secondary antibodies conjugated to fluorescent dyes have worked well for us over the years. They have a long storage life, high specificity, great signal:noise ratio and are reasonably priced in comparison to other vendors."

"I use this product on a daily basis and it is excellent and working very well"

"Excellent product and working well. Some nonspecific staining has been detected with the donkey raised Ab."

"The secondary antibodies are easy to use and are fairly sensitive. I would recommend using them at the standard dilution of 1:100 to 1:200. After sales care was fine. It would have been nicer to buy several vials of the same lot after testing efficiency instead of being sent different lots each time you order. The value is comparable to other companies but if the antibody were a bit more sensitive and could be used at a more dilute concentration, it would be ideal."

"Without question, I can always turn to Jackson ImmunoResearch secondary antibodies for ECL detection of any primary antibody! They produce great signal at low dilutions, last for years even through bad laboratory practice like repeatedly using the same tube every time, and come in a variety of species flavors. Special shout-out to the light-chain specific secondaries!"

"I have been using the HRP-conjugated Whole IgG Affinity-Purified Secondary Antibodies for years now. I appreciate the variety of antibodies available and the minimum cross-reactivity factor. As well, new bovine HRP prove to be very useful for antibodies giving high background (mostly the ones raised in Goat for my work)."

"Product works well as a control for our experiments."

"Easy to use, very high quality product and I would definitely recommend these products to other researchers. We only use secondary antibodies from Jackson ImmunoResearch whenever possible! We won't use another company. Customer service is also excellent and the price point for their products is very competitive."

"Work always very well. Decent selection of fluorophores."

"This product was a great purchase for our lab as we looked to conduct studies using secondary antibodies"

"I've been using Jackson ImmunoResearch Secondary Antibodies since my graduate school days (33 years!) in my group's ELISAs and FACS work. Their reliability/consistency is outstanding. It is great to have a "go-to" vendor for this crucial component of many immunoassays."

"I have tried three different species and all work well"

"Jackson's secondary antibodies are very easy to use. I use them diluted 1:100 in PBS. They give great and trustworthy results. I would strongly recommend them to other scientists. They are not very expensive and in my opinion they are value for money. Customers service is also great, they respond quickly to my inquiries."

"All the secondary antibodies we ordered from Jackson ImmunoResearch always performed flawlessly; when diluted in glycerol as instructed they last for years at -20c."

"The antibodies I used were FITC-anti mouse IgG and Cy3-anti rabbit IgG. Both of them worked well, the signals were clean with no background due to nonspecific binding. The antibodies are easy to use - they require only to be diluted. No special after sales care is required - only to keep them at the right temperature. As with many scientists, our lab also struggles to have enough money in order to fulfil our scopes. My rating 4/5 stars for "value for money" doesn't mean that the value is low, it simply means that the price is high. Off course I understand that it is not so easy to lower the price and to keep the same quality. "

"We used secondary antibody from Jackson ImmunoResearch Laboratories, Inc and published our paper in Neurobiology of Disease, http://www.sciencedirect.com/science/article/pii/S0969996115001643 Thanks SelectScience for offering useful products review and helping us make a decision before purchase!"

"Secondaries from Jackson always give me great results."

"Pretty user friendly and quick to use. It is definitely worth the money spent when compared to the time I saved."

"I've been using the HRP-conjugated whole IgG affinity purified secondary antibodies for over 10 years for ECL detection in immunoblotting. These antibodies give consistent and reproducible results in multiple types of blocking solutions (i.e. milk, BSA, I-BLOCK, etc.). A major advantage is that the antibodies arrive as lyophilized powders which allows you to control the expiration date once constituted. Additionally, HRP activity remains detectable when stored at 4C short-term, -20C in 50% glycerol (6 mo-1 year) or -70C+ long-term. It is best to work from aliquots of reconstituted antibodies."

"These antibodies were very easy to use. We coupled them with specific probes and the experiments worked well. I would recommend this product for people who want to perform PLA."

"Great customer support and very knowledgeable people. Secondary Ab's I purchased were of great quality."

"It was easy to use and got better results. It seemed cost effective too. Initially I was having some problems which was taken care of by the technical person. I was pleased with their performance."

"I have used a wide variety of secondaries and Jackson ImmunoResearch has consistently been the best. The fluorophores are bright and stable and their selective (x reactivity removed) secondaries have always shown species specificity in multiple labeling. Not the cheapest, but the best."

"High quality products produced by the most trustworthy company."

"Jackson products are excellent. I have been using Jackson products for more than 20 years and I always recommend them to my colleagues. Very easy to use and reliable. Very good value for money. Using Jackson secondary antibodies always produces excellent labeling and fluorescent images which resulted in having 4 covering pages for 4 prestigious international journals."

"It is a good product, easy to use and gives good results. I recommend it."

"Jackson make some of the best secondaries around. They are very easy to use and inexpensive. My biggest problem is that Jackson don't have every variety that we need. We use a lot of different animal types and need several different methods for detecting them. Sometimes we end up having to buy our secondaries through Fisher because of this. "

"Good product! It is easy to use and the signal/ noise is low. Great value for the money!"

"We have used secondary antibodies for many years now. Their products are extremely reliable and give reproducible results. We have not had any difficulties with antibodies from Jackson immunoresearch and we use a variety of secondary antibodies in the lab."

"Very clean secondaries, find that if I see background, it is due to a primary problem. Would love to see you Jackson add the BV 421 and BV 480, preferably in Donkey against all the other species!"

"We use this product for screening and confirmation of expression."

"The reagents are very easy to use. Data generated is of high quality. I definitely recommend the products to other scientists."

"Jackson Immunoresearch has high quality reagents with secondary antibodies tags with a variety of chromophores."

"After years of use of Jackson's secondary antibodies, I am still entirely satisfied and I will continue to use those products that totally fulfill my needs."

"The secondary antibody IgGs, such as goat anti-human, donkey anti-mouse and so on, are very reliable. They gave the strong IHC positive expression and less background. Another benefit is they have the big volume. Usually they were diluted to lower concentration. It means you can use them for a long time. Their stability can be trusted too."

"The quality of these antibodies is extremely high and is our first choice. The labels are extremely accurate to how the antibody is generated and if it is cross absorbed and or labeled."

"Usually high-quality results. Would recommend to others. Have to be diluted 1+1 with glycerol. "

"I have used Whole IgG Affinity-Purified Secondary Antibodies from Jackson labs. They are just awesome. Very precise and accurate. Easy to use. Excellent customer service."

"It is a good product from Jackson ImmunoResearch Laboratories. We have a nice experience. I strongly recommended this product from Jackson ImmunoResearch Laboratories."

"These are great products! I use all kinds of IgG purified secondary antibodies and never seem to have any secondary-related problems! I have not actually used customer service in quite some time, but I suspect that they deserve 4 stars!"