UltraScript® Reverse Transcriptase
UltraScript® Reverse Transcriptase is a robust and thermostable modified MMLV reverse transcriptase engineered to enhance cDNA synthesis speed and yield with accurate transcript representation.
Reliable reverse transcription
Unbiased representation of mRNA ends
Broad dynamic range of reverse transcription
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Results are very good, I would like to use it again
For nascent RNA expression
Easy to use, need less time than other similar products and no obvious difference from other products.
Review Date: 12 Jan 2024 | PCR Biosystems
Great results
Screening
The product is excellent in reverse transcription with many RNA viruses like PPRV and produce good results. It is easy to use with good results
Review Date: 8 Jun 2021 | PCR Biosystems
UltraScript® Reverse Transcriptase is a thermostable and extremely active modified MMLV reverse transcriptase (RTase) for cDNA synthesis. The reaction temperature can be increased up to 55 °C providing higher specificity and efficient transcription of GC-rich RNA regions with a high secondary structure. The RTase is blended with an advanced RNase inhibitor preventing degradation of RNA by contaminating RNase. UltraScript® Reverse Transcriptase is not inhibited by ribosomal and transfer RNAs, making total RNA an ideal substrate.
The enzyme is supplied with a 5x buffer containing Mg, dNTPs, stabilizers and enhancers, optimised to generate high yield cDNA from as little as 4 pg RNA. As oligos are not included, UltraScript® Reverse Transcriptase provides the convenience and flexibility for users to define their own priming strategy depending on the type of analysis needed. UltraScript® Reverse Transcriptase gives exceptional performance with gene-specific primers, oligo(dT) and random hexamers to produce high quality cDNA ideal for a variety of downstream applications.
Features
- Unbiased representation of 5’ and 3’ mRNA transcript ends
- Reduced RNase H activity
- Thermostable reverse transcriptase 45 °C to 55 °C
- Advanced RNase inhibitor
- High cDNA yields from as little as 4 pg total RNA
- Accurate reverse transcription of GC-rich templates
- Sensitive detection of low copy number transcripts
- Advanced buffer chemistry including Mg and dNTPs
- Both premixed or separate oligo cDNA synthesis kits available
