The cellular microenvironment is composed of extracellular matrix (ECM) proteins, surrounding cells, cytokines, growth factors, hormones, and other bioactive agents. Traditional 3D cell cultures rely on encapsulating cells in tumor-derived or synthetic hydrogels that do not fully recapitulate the native tissue microenvironment and can lead to variable and misleading experimental results.
To isolate the native extracellular matrix of a tissue from its inhabiting cells, a decellularization process is employed. The resulting decellularized extracellular matrix (dECM) scaffolds can be used in cell culture and tissue engineering applications. These dECM hydrogel scaffolds can provide a more physiological environment with increased growth rates for cells without the use of any exogenous growth factors.
Our tissue-specific dECM Gel Hydrogel Kits include a series of native decellularized extracellular matrices (dECMs) derived from a variety of non-diseased porcine organs (bone, heart, liver, kidney, intestine, skin and lung). These hydrogels can be used as traditional 2D ECM coatings or to encapsulate cells for 3D cell culture applications, with enhanced performance compared to other hydrogels.