Status:
Advanced ReviewerMember since: 2019
Organization: University of Chicago
Remarkably simple to use and great results in only minutes.
Application Area: Synthetic molecular biology
"The Rapid DNA ligation kit is extremely efficient with a seamless reaction set up with the entire process taking less than 30 minutes. For more challenging reactions we do extend the reaction time to an hour. Moreover, we reduce the reaction volume to 25% of the stated protocol and have excellent results nearly every time. Great value for the cost."
Status:
Advanced ReviewerMember since: 2013
Organization: Umass Medical School
Great product
Application Area:Molecular Cloning
"This kit is really easy to use and never fails. I get plenty of colonies every time."
Thermo Scientific Rapid DNA Ligation Kit enables fast sticky-end or blunt-end DNA ligation in only 5 minutes at room temperature.The kit contains T4 DNA ligase and a specially-formulated 5X rapid ligation buffer optimized for fast and efficient DNA ligation. Fast ligation efficiency is equal to that obtained with T4 DNA ligase in a standard 1 hour ligation. The ligation reaction mixture can be used directly for bacterial transformation with the TransformAid Bacterial Transformation Kit or with other conventional transformation procedures.
Manufacturer Thermo Fisher Scientific | Available Worldwide
5.0 / 5.0 | 2 reviews