Member since: 2020
Organization: University of Copenhagen
Great instrument that made my PhD research possible.
Application Area: Functional screening of ion channel variants (ASIC1a)
"I had the chance to use the SyncroPatch for parts of my PhD research, enabling the high-throughput functional characterization of over 300 different ASIC1a variants. Working with transiently transfected cells that were FACS-sorted before the patch-clamp experiments, I didn't expect perfect seals, but I still got good quality data and reproducible results that formed the basis for my PhD project. I measured CRCs for activation and steady-state desensitization, as well as peptide modulation of the channel, and got fantastic support from Søren Friis both with technical questions and assay design. The SyncroPatch and its software are easy to use and allow for versatile assay design. The team at Nanion goes out of their way to help with all questions and requests that come up, and they host fantastic user meetings for idea exchange. After four years using the SyncroPatch, I can highly recommend it."
The SyncroPatch 384i is a high performance automated patch clamp (APC) system for recording from up to 384 wells simultaneously, with the possibility of upgrading to an unprecedented 768 wells. The heart of the SyncroPatch 384i, the patch clamp module designed and produced by Nanion Technologies, is integrated into the state-of-the-art liquid handling robot, the Biomek i5, from Beckman Coulter.
Capable of recording up to 20,000 data points per day per module, the SyncroPatch 384i bridges the gap between patch clamp and high throughput screening. But the SyncroPatch 384i is much more than a high throughput screening instrument: It can be efficiently integrated into all drug discovery phases including smaller research tasks and biophysical studies, taking advantage of the high data quality, low cost per data point and the ability to perform partial plate measurements which is particularly useful in assay development or for smaller screens.