Member since: 2023
Organization: Assay.Works GmbH
SURFE2R has proved itself as a valuable asset, providing robust and reproducible data
Application Area: SSM-based Electrophysiology Screening Platform
"SURFE²R 96SE is a unique platform and favors some exceptional and astonishing capabilities. Employing solid-supported membrane (SSM)-based electrophysiology, SURFE²R 96SE can resolve small currents of transporters and pumps in a high-throughput format that makes it ideal for screening assays. In our hands, SURFE2R 96SE has proved itself as a valuable asset, providing robust and reproducible data. Using SURFE2R 96SE, it is much more feasible to conduct concentration response screening in an industrial setting. Some targets such as organelle transporters that can be readily and in high-throughput format studied using SURFE²R 96SE, are inaccessible for other techniques."
Member since: 2020
Our "Gold Standard" as secondary assay system for electrogenic transporters.
Application Area:Drug discovery, secondary transporter assays
"The SUERFE2R is our "Gold Standard" as a secondary assay system for electrogenic transporters. Some experience is needed, as usual, for these kind of assay systems, but the instrument is easy to use and data evaluation is easy to conduct. The assay and technical support is superb."
The SURFE²R 96SE is designed for activity measurement of electrogenic membrane transporters and pumps, which generate currents too low to resolve by patch clamp or which are not expressed in membranes accessible for patch clamp (e.g. bacterial, organelles). The technology enables label- and radioactivity-free real time measurements with high time resolution.
The SURFE²R 96SE uses sensors in a standard 96-well plate format. Membranes containing the protein of interest are adsorbed onto these sensors. Any substrate can be added to activate the transporter. The resulting transport or binding currents will be detected and analyzed accordingly.
The whole process, from sample preparation and measurement to data analysis, is fully automated.