Use StarBright Blue 700 Goat Anti-Rabbit IgG for ultra-sensitive detection of proteins in fluorescence western blotting. This secondary antibody is ideal for use in multiplex blotting, eliminating the need for stripping and reprobing, or cutting blots. StarBright Blue 700 Goat Anti-Rabbit IgG is optimized for use with the ChemiDoc™ MP Imaging System, permitting detection of up to three proteins in a single blot.
The StarBright Fluorophore is composed of multiple monomers that each absorb and emit light, giving a higher quantum yield than other fluorophores and creating a very strong signal. Additionally, the large separation between Exmax and Emmax (470/700 nm) results in minimal spectral overlap between emission and excitation wavelengths.
Schematic representation of a StarBright Label conjugated to an antibody. The light-absorbing monomer is excited at 470 nm and, via an efficient intra-molecular energy transfer process, the energy is transmitted to a light-emitting monomer which emits at 700 nm. The linker monomer is used for antibody conjugation.
StarBright Blue 700 Secondary Antibodies can be used for multiplex fluorescent western blotting with the right combination of fluorophores. These fluorescent secondary antibodies can also be used to detect post-translational modifications like phosphorylation of a target protein.
Note: StarBright Antibodies are not suitable for stripping and reprobing.