The new FPLC column product line offers new high-quality alternatives for Size-Exclusion (SEC), Affinity (AC) and Ion-Exchange chromatography (IEX). The FPLC column line is completing the KNAUER FPLC portfolio and provides the best LC column addition to your FPLC system.
With these materials, the FPLC column Sepapure is providing the best matching columns to your precious AZURA FPLC system, giving you the complete and reliable KNAUER FPLC package.
The FPLC columns come as prepacked ready-to-use 1 ml or 5 ml cartridge and can also be purchased as bulk resin. The media for affinity chromatography as well as ion-exchange chromatography is based on agarose, which is a well-known in FPLC chromatography. For affinity chromatography the agarose solid phase is functionalized with ligands for purification of recombinantly expressed proteins with His- or GST-tags or antibodies via Protein A or Protein G affinity. The Ion-Exchange columns can be purchased as weak and strong anion or cation exchangers. The basis of our FPLC desalting column is dextran, which reliably separates small molecules and salts from valuable proteins.