Member since: 2014
Organization: Cascade biosystems
Application Area: Medical testing
"Very good tool for fast testing. very accurate, affordable and easy to use. Highly recommended."
It is a double antibody (sandwich) ELISA using a polyclonal anti-rotavirus antibody to capture the antigen from the stool supernatant. A second anti-rotavirus monoclonal antibody is then added, which binds to the complex. This reaction is visualized by the addition of anti-mouse antibodies conjugated to peroxidase. The resulting blue color, following the addition of the chromogen and peroxide, indicates the presence of rotavirus antigens being bound by the anti-rotavirus antibodies.