Recombinant Human TNF-alpha/TNFSF1A by R&D Systems, Inc.

Manufacturer R&D Systems, Inc.
4.3
/
5.0
  |  1 reviews
R&D TNF-alpha / TNFSF1A - Recombinant Human Protein Tumor necrosis factor alpha (TNF-alpha), also known as cachectin and TNFSF1A, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, apoptosis, and immune system development. Specifications and Use for R&D TNF-alphaSource of R&D TNF• A DNA sequence encoding the mature human TNF-α protein sequence (Accession # X01394) was expressed in E. coli. Molecular Mass of R&am... Read more


Recombinant Human TNF-alpha/TNFSF1A by R&D Systems, Inc. product image
Recombinant Human TNF-alpha/TNFSF1A

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Average Rating: 4.3
1 Scientist has reviewed this product

4 out of 5
Ease of use
4 out of 5
After sales service
5 out of 5
Value for money


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    Reviewer
  • Member since: 2017

  • Organization: IDIBAPS



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
Excellent quality/price ratio!
Rating: 4.3

  • Application Area: stimulate cells in culture to mimic inflammatory environment

"The recombinant proteins from Peprotech are of high quality in terms of reproducibility and at the same time they're affordable. I trust Peprotech's cytokines and growth factors for my "picky" glioma stem-like cells. They are also fast suppliers. I've been a satisfied customer since I started my lab back in 2010."

Review date: 20 Dec 2017 | Recombinant Human TNF-alpha/TNFSF1A
R&D TNF-alpha / TNFSF1A - Recombinant Human Protein

Tumor necrosis factor alpha (TNF-alpha), also known as cachectin and TNFSF1A, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, apoptosis, and immune system development.

Specifications and Use for R&D TNF-alpha

Source of R&D TNF
• A DNA sequence encoding the mature human TNF-α protein sequence (Accession # X01394) was expressed in E. coli.

Molecular Mass of R&D TNF
• The purified recombinant human TNF-α has a molecular mass of approximately 17.5 kDa. The recombinant protein preparation is a mixture of a 158 amino acid residue form containing an N-terminal methionine and a 157 amino acid residue form with the authentic sequence of mature human TNF-α.

Purity of R&D TNF 
• > 97%, as determined by SDS-PAGE and visualized by silver stain.

Endotoxin Level
• < 1.0 EU per 1 μg of the cytokine as determined by the LAL method.

Activity of R&D TNF-alpha
• Measured in a cytotoxicity assay using the TNF susceptible murine L-929 cell line in the presence of the metabolic inhibitor actinomycin D (Matthews, N. and M.L. Neale in Lymphokines and Interferons, a Practical Approach, 1987, M.J. Clemens, A.G. Morris and A.J.H. Gearing, eds., IRL Press, p. 221).
♦ The ED50 for this effect is typically 25 - 100 pg/mL in a fluorometric assay using the redox senstitive dye, Resazurin (R&D Systems, Catalog # AR002).

Formulation of R&D TNF
• Lyophilized from a 0.2 μm filtered solution in PBS containing 50 μg of bovine serum albumin per 1 μg of cytokine.
• It is recommended that sterile phosphate-buffered saline containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of 100 μg/mL of the cytokine.

Reconstitution of R&D TNF
• Lyophilized samples are stable for up to twelve months at -20° C to -70° C.

Storage of R&D TNF-alpha
• Upon reconstitution, this cytokine can be stored under sterile conditions at 2° - 8° C for one month or at -20° C to -70° C in a manual defrost freezer for three months without detectable loss of activity.
• Avoid repeated freeze-thaw cycles.