QIAxcel DNA High Resolution Kit (1200)
For automated analysis of DNA fragments using QIAxcel instruments
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QIAxcel instruments, when used in conjunction with QIAxcel DNA Kits, provide fully automated size separation and quantification of DNA fragments in up to 96 samples per run. QIAxcel technology, based on capillary electrophoresis using gel cartridges, provides unmatched resolution, speed, and throughput. QIAxcel gel cartridges are reusable, allowing up to 250 runs of 12 samples to be performed. Preinstalled methods suitable for most applications are provided. QIAGEN can also create customized methods for users.
The QIAxcel DNA Kits are only used in combination with the QIAxcel Advanced System and the QIAxcel DNA Accessories.
Identifying Rare Mutations in Diamond-Blackfan Anemia Using Target Enrichment and Next-Generation Sequencing
Diamond-Blackfan anemia (DBA) is a rare congenital stem cell disorder associated with monoallelic inactivating mutations in the ribosomal protein (RP) genes. It leads to bone marrow failure syndrome by causing defects in erythroid progenitor and precursor cell development. Loss of function mutations in 10 of the c. 80 RP genes have been definitively associated with DBA. RPS19 is mutated in up to 25% of DBA cases, and 13 other RP genes are mutated in a further 25–35%. The molecular basis of the remaining 40–50% of cases is unknown. Since such cases may harbor mutations in one or more of the remaining RP genes and such mutations may occur at very low frequencies, genetic screening using conventional Sanger sequencing on a perexon/per-gene basis is challenging.
Fish Species Identification Using PCR-RFLP and the QIAxcel Advanced
Despite a long history of regulation to maintain a healthy fish industry, mislabelling of fish species sold on the market still exists. Typically, PCR-RFLP (restriction fragment length polymorphism) is used for the identification of fish species, however this is a time-consuming method and requires special equipment. In this application note, the QIAxcel native capillary electrophoresis system as an alternative to conventional gel electrophoresis.
