Member since: 2018
Organization: Brigham and women's hospital
Excellent plasmid extraction kit.
Application Area: Plasmids extraction
"Really easy to use. All of the procedures can be done in one and a half hours. The quality of the plasmids are pretty good, even 260/280 ratio is always around 1.9, but that would not affect transfection efficiency too much."
Member since: 2009
Organization: Dart NeuroScience LLC
Application Area:Endotoxin-Free Plasmid DNA Preparation
"1. The column is very easy to use. 2. It can obtain high quality plasmid DNA, easy to use for transfection. 3 It can process 20-30 samples same-time, at high efficiency. 4. Only negative point is the results are inconsistent. I called tech support, they said can't use more than 20 ml of culture, But in the past, I got very high yield even from 100 ml of cultured bacteria and sometimes failed to get DNA from 20 ml of culture."
QIAGEN Plasmid Plus Kits
For the fastest, most convenient purification of up to 10 mg transfection-grade plasmid DNA
Qiagen Plasmid Plus Midi Kit, 100 preps, 25 to 50mL Culture Volume, Transfection-grade Plasmid or Cosmid DNA Purification, Advanced Silica Technology, Manual Processing, 20 min. Time/Run, Up to 1mg Yield, For the Fastest, Most Convenient Purification of up to 250μg Transfection-grade Plasmid DNA, Ideal Transfection of most Cell Lines, Cloning, Enzymatic Restriction, Includes 100 Qiagen Plasmid Plus Midi Columns, Extender Tubes, Reagents, Buffers, 100 QIAfilter Midi Cartridges