Status:
ReviewerMember since: 2020
Organization: University of Szeged, Hungary
Reliable instrument and fantastic support.
Application Area: Ion channel pharmacology
"The Port-a-Patch is a very compact single-channel bench-top workstation. Chip mounting station and amplifier head-stage are integrated in the shielded device, while an additional small suction control unit, a patch-clamp amplifier and a computer complete the system. However, it was further miniaturized by integrating the amplifier into the system in case of Port-a-Patch mini. Using this semi-automated patch clamp system is very easy. The user just pipettes solutions and cells onto the disposable recording chip, where a cell is automatically captured and sealed using the suction control unit. Visual control of this process by microscope and micro-manipulator are not required. Many add-ons, such as temperature controlled external perfusion system or the unique internal perfusion system expand the range of possible experiments can be performed by this device. Very impressive technology and system for quick test of cells and ion channels. However, for higher throughput, I recommend the Patchliner or SyncroPatch from Nanion, where 8 or 384 wells are recorded in parallel, respectively. In addition to state-of-the-art and reliable instruments, the experienced team at Nanion provide exceptional support and technical background to develop the experimental methods and achieve the best quality records."
Status:
Advanced ReviewerMember since: 2013
Organization: National Laboratory of Channelopathies
High quality results, comparable in all respects to manual classic patch-clamps.
Application Area:Electrophysiological patch-clamp recording of ion channel functional activity
"High quality an reproducible results. Easy to use and very effective and reliable attention from the manufacturer after purchase. Highly recommendable technology and instrument."
Status:
ReviewerMember since: 2020
Organization: National Laboratory of Channelopathies
Reliable and powerful data production
Application Area:Patch-clamp of ion channel activity
"Reliable and powerful data production"
Status:
ReviewerMember since: 2010
Organization: Cornell University
"Excellent easy to use system, no in depth knowledge of patch clamp technologies necessary. Excellent for screening and short characterization protocols."
Using the Port-a-Patch is straightforward and easy – the user simply adds solutions and cells onto the disposable recording chip, where a cell is automatically captured and sealed by suction using a computer controlled pump. A microscope or micro-manipulator are not required.
Add-Ons such as the Internal and External Perfusion and Temperature Control, as well as the ability to conduct current clamp and bilayer experiments, broaden the range of possible experiments for scientific research immensely.
The Port-a-Patch system is comprised of the Port-a-Patch recording unit, a Suction Control unit, a HEKA amplifier and a computer. If an amplifier is already available, this can be integrated with the Port-a-Patch system in most cases. Further add-ons are available for specific assays as e.g. temperature control, microscope slides or automated perfusion systems.
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