Status:
Reviewer
Member since: 2014
Organization: Walther-Straub-Institut
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- Ease of use
- After sales service
- Value for money
Great support, easy t Highly recommended
Application Area: Automated electrophysiology for TRP-research (TRPA1; TRPV4)
"In 2019, we have been ordering yet another Port-a-Patch and we did not regret doing so. The instrument was ordered to supplement and enhance our research in the field of TRP-channels under toxicological circumstances. Our needs were as following: 1) Small setup to be placed under a fume hood 2) Small dead volumes to keep the toxic waste low 3) "Easy to apply" setup 4) No preparation is needed. You can go from zero to measurement in about 10-15 min. The system is built for nonelectrophysiologists as no excessive previous knowledge is necessary (this doesn't mean that it won't be an advantage to know what you are doing)! The port-a-patch did tick all these boxes and in addition, you receive extraordinary, helpful and experienced support from Nanion. Especially small adjustments regarding the protocol for a special cell type are covered very well by the "Nanion-database". It did safe us plenty of time as Nanion might very well have already dealt with a similar question. In this regard we did never lose much time establishing the method, instead, we could immediately start measuring. The measurements themselves have proven reliable, repeatable, and very sensitive. As we were coming from a Ca2+-based method, we were pleasently surprised that our stimulus was more effective than expected. In my opinion, the Port-a-Patch (or Portie) is unmatched as it allows to overcome many hurdles that regular electrophysiology brings while being smaller and easier to use. If you are looking for higher throughput i can as well recommend having a look at the patchliner or synchropatch."
Review date: 22 Nov 2021 | Port-a-Patch
Status:
Reviewer Member since: 2020
Organization: National Laboratory of Channelopathies
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- Ease of use
- After sales service
- Value for money
Best instrument in its class
Application Area:Recording of ion channel functional activity by the automated patch-clamp technology
"Highest possible quality results obtained with this instrument"
Review date: 23 Jun 2021 | Port-a-Patch
Status:
Reviewer Member since: 2020
Organization: University of Szeged, Hungary
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- Ease of use
- After sales service
- Value for money
Reliable instrument and fantastic support.
Application Area:Ion channel pharmacology
"The Port-a-Patch is a very compact single-channel bench-top workstation. Chip mounting station and amplifier head-stage are integrated in the shielded device, while an additional small suction control unit, a patch-clamp amplifier and a computer complete the system. However, it was further miniaturized by integrating the amplifier into the system in case of Port-a-Patch mini. Using this semi-automated patch clamp system is very easy. The user just pipettes solutions and cells onto the disposable recording chip, where a cell is automatically captured and sealed using the suction control unit. Visual control of this process by microscope and micro-manipulator are not required. Many add-ons, such as temperature controlled external perfusion system or the unique internal perfusion system expand the range of possible experiments can be performed by this device. Very impressive technology and system for quick test of cells and ion channels. However, for higher throughput, I recommend the Patchliner or SyncroPatch from Nanion, where 8 or 384 wells are recorded in parallel, respectively. In addition to state-of-the-art and reliable instruments, the experienced team at Nanion provide exceptional support and technical background to develop the experimental methods and achieve the best quality records."
Review date: 01 Jan 2021 | Port-a-Patch
Status:
Advanced Reviewer Member since: 2013
Organization: National Laboratory of Channelopathies
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- Ease of use
- After sales service
- Value for money
High quality results, comparable in all respects to manual classic patch-clamps.
Application Area:Electrophysiological patch-clamp recording of ion channel functional activity
"High quality an reproducible results. Easy to use and very effective and reliable attention from the manufacturer after purchase. Highly recommendable technology and instrument."
Review date: 05 Jun 2020 | Port-a-Patch
Status:
Reviewer Member since: 2020
Organization: National Laboratory of Channelopathies
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- Ease of use
- After sales service
- Value for money
Reliable and powerful data production
Application Area:Patch-clamp of ion channel activity
"Reliable and powerful data production"
Review date: 26 Feb 2020 | Port-a-Patch
Status:
Reviewer Member since: 2010
Organization: Cornell University
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- Ease of use
- After sales service
- Value for money
"Excellent easy to use system, no in depth knowledge of patch clamp technologies necessary. Excellent for screening and short characterization protocols."
Review date: 08 Dec 2011 | Port-a-Patch
Using the Port-a-Patch is straightforward and easy – the user simply adds solutions and cells onto the disposable recording chip, where a cell is automatically captured and sealed by suction using a computer controlled pump. A microscope or micro-manipulator are not required.
Add-Ons such as the Internal and External Perfusion and Temperature Control, as well as the ability to conduct current clamp and bilayer experiments, broaden the range of possible experiments for scientific research immensely.
The Port-a-Patch system is comprised of the Port-a-Patch recording unit, a Suction Control unit, a HEKA amplifier and a computer. If an amplifier is already available, this can be integrated with the Port-a-Patch system in most cases. Further add-ons are available for specific assays as e.g. temperature control, microscope slides or automated perfusion systems.
Features:
- External & internal perfusion
- Temperature control
- Voltage & current clamp
- Whole-cell & perforated patch clamp
- Chips produced in-house, various resistances available (customizable on request)
- Rseries compensation
- Unlimited compound applications
- Voltage and ligand-gated ion channel analysis
- Cell lines, primary cells and stem cells can be used
- Minimal training required
- Adjustments of parameters during experiments possible for fast and efficient assay development
Product Overview
