PolyFreeze Tissue Freezing Medium by Merck KGaA, Darmstadt, Germany

PolyFreeze is a support matrix, or form of embedding medium, for frozen tissue sectioning. This medium freezes quickly, supporting the tissue for sectioning at 3 µ and up with no cracking of the matrix at temperatures from –8 to –25 C. Tissue samples may be snap frozen using PolyFreeze with isopentane and liquid nitrogen, dry ice, or in a cryostat.PolyFreeze media can easily be washed away when rinsed prior to staining and leaves no residue to interfere with staining or immunohistochemistry (IHC) reactions. Does not create autoflorescence.