NightSHADE evo LB 985N In Vivo Plant Imaging System

Delayed fluorescence acts as an indicator not only for chlorophyll content, but also for the physiological state of the plant which can vary based on environmental influences such as drought, high saline levels or mycotic infections. This application note demonstrates the use of delayed fluorescence for in vivo imaging of plants as a monitoring system for stress factors.

Biophoton emission (BE) or autoluminescence imaging is a method to measure stress status of plants in a non-invasive way similar to delayed fluorescence. Both are extremely weak light emissions which can be used to monitor the physical state of a plant. This application note shows the difference between both methods and state parameters to perform these measurements using the NightSHADE plant imaging system.

The usage of fluorophores such as YFP and GFP is a widely distributed and established method in plant research. To monitor fluorescent reporter proteins several techniques based on instruments such as fluorescent microscopes or imaging systems are available. Detection of free or fused fluorescent reporters in different subcellular localizations and with different expression levels can be performed with the NightSHADE LB985 imaging system. The obtained results, presented in this application note, correlated with data received by detection of fluorescent using the Mithras LB940 multimode plate reader.

In this application note by BERTHOLD TECHNOLOGIES, gene expression in Arabidopsis is analysed using promoter::firefly Luciferase (LUC) reporter constructs and the NightSHADE imaging chamber. Analysis of circadian clock associated 1 (CCA1) and chlorophyll a/b-binding protein (CAB) constructs showed that CCA1 expression preceded activation of CAB promoter, consistent with CCA1 protein playing a positive role in CAB gene expression.

Watch this video to learn about the new TriStar², a budget multimode microplate reader, that works with luminescence, fluorescence, and absorbance technologies. The Mithras², a high-end multimode reader, incorporates a monochromator which enables for flexibility in wavelength selections. Bernd also discusses the NightSHADE, an innovative solution to in vivo imaging in plants. Interview filmed by SelectScience at analytica 2014.

Learn about the Nightshade LB985 for long term observation of gene expression in this interview from Biotechnica. Bernd Hutter from Berthold describes the Nightshade, which enables in vivo imaging for plant research and combines both a growth chamber and imaging system in one instrument. The instrument has a sensitive CCD camera enabling the plants to be imaged from the side, observing the geotropic aspects which allow the long term observation of gene expression and analysis. Biotechnica 2011