NADH by Merck

Manufacturer Merck  |  Available Worldwide
4.7
/
5.0
  |  1 reviews
Grade I, disodium salt


NADH by Merck product image
NADH

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Average Rating: 4.7
1 Scientist has reviewed this product

5 out of 5
Ease of use
4 out of 5
After sales service
5 out of 5
Value for money


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    Reviewer
  • Member since: 2016

  • Organization: Ibex Pharmaceuticals inc.



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
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Rating: 4.7

  • Application Area: Enzyme activity measurement

"NADH reduced in 5 mg vials is very convenient. Easy to use. I have never had a problem with Sigma-Aldrich products."

Review date: 01 Aug 2017 | NADH

Absorbance coefficients: 14.3 x 103 l x mol-1 x cm-1 at 260 nm, 6.18 x 103 l x mol-1 x cm-1 at Hg 334 nm, 6.3 x 103 l x mol-1 x cm-1 at 340 nm, 3.4 x 103 l x mol-1 x cm-1 at Hg 365 nm

General description

For life science research only. Not for use in diagnostic procedures.

Storage conditions (working solution): 10 mg/ml in Na-bicarbonate buffer, pH 9, contains at least 95% NADH after 4 weeks at 2 to 8 °C.

Contaminants: Relative reaction with LDH: 1.00 ± 0.05 (referred to a standard)

Organic solvents: <4% (GC)

Application

As a reagent, NADH can be used in enzyme cycling assays to amplify detection of activity of biologically relevant enzymes or metabolites present in low concentrations.

Biochem/physiol Actions

NADH is a coenzyme that functions as a regenerating electron donor in catabolic processes including glycolysis, β-oxidation and the citric acid cycle (Krebs cycle, TCA cycle). It participates in cell signaling events as well, for example as a substrate for the poly (ADP-ribose) polymerases (PARPs) during the DNA damage response. The NAD+/NADH dependent sirtuins play key roles in stress responses during events involving energy metabolism, with implications in cancer biology, diabetes and neurodegenerative disease.
As a reagent, NADH can be used in enzyme cycling assays to amplify detection of activity of biologically relevant enzymes or metabolites present in low concentrations.

form   amorphous powder
mol wt   Mr 665.4 (NADH)
  Mr 709.4 (NADH-Na2)