iQue QBeads® PlexScreen
Bead Based Assays
Receive your quote directly from the manufacturer.
iQue QBeads® are a family of reagents that facilitate the capture of specific proteins on distinct bead types, enabling multiplexed quantitation of cytokines, adhesion molecules, enzymes, growth factors receptors, and more.
The iQue QBeads® PlexScreen family of reagents are custom built into complete ready-to-run kits. The kits consist of beads to capture human, mouse or rat secreted proteins, detection reagents, standard protein, buffers, and analysis templates.
Each analyte has been validated in no-wash and 1-wash protocols. Key Benefits:
- No wash protocol, just add and read for fast, easily automated workflow.
- Custom-built to your order; we maximize your flexibility!
- Available for human, mouse, and rat secreted proteins including cytokines, chemokines, enzymes, and growth factors.
- Analyze up to 30 analytes simultaneously, with no reduction in analysis speed.
- Multiplex with other iQue® kits like Cell Membrane Integrity for richer content
Multiplexed Detection of Mouse Cytokines Using QBeads and the iQue Screener
The use of rodent QBeads® Kits with the iQue® Screener enables multiplexed quantification of secreted mouse or rat cytokines in high throughput screening applications. Together, QBeads and the iQue Screener allow dramatic assay miniaturization in microtiter plates, reducing reagent costs as well as minimizing the required amount of expensive or difficult-to-obtain cell types. QBeads’ no-wash assay protocol combined with the iQue Screener’s rapid sampling technology allow more samples to be analyzed in less time, making it practical to screen larger libraries, test more doses, and observe more time points.
Application of MultiCyt QBeads for High Throughput Cytokine Profiling in Human Peripheral Blood Mononuclear Cells
Aberrant production and/or secretion of proteins have long-reaching effects for physiological processes, and dysfunctional signaling is implicated in virtually all diseases. The detection and quantification of proteins in solution provides key information for researchers in therapeutic areas ranging from cancer and immune function to aging and regeneration. In the case of immune function, an appropriate response involves a coordinated set of signals across multiple cell types and the regulation of such responses depends on cytokine-dependent cell-to-cell communication. These complex signaling pathways between cells are an important target across the drug discovery process, from primary screening to toxicity profiling. This application note describes how cytokine profiling in human blood cells can be carried out using Intellicyt's MultiCyt QBeads.
Advancing Therapeutic Antibody Discovery with Multiplexed Screening
Antibody-based therapeutics are the fastest growing and most successful therapeutic modality for treating diseases such as cancer, cardiovascular disease, autoimmune disorders and infectious disease.This whitepaper describes how the iQue Screener is used to perform multiplexed screens for antibody binding to either cell surface or to circulating target antigens. The whitepaper also discusses the use of the same platform to carry out high content assays to evaluate the effects of lead candidates in multiplexed cell based and secreted protein assays.
