Mouse Inflammation Array C1 by RayBiotech Inc.

Manufacturer RayBiotech Inc.  |   Model: AAM-INF-1
RayBio® C-Series Mouse Inflammation Antibody Array 1 Kit. Detects 40 Mouse Inflammatory Factors. Suitable for all liquid sample types.


Mouse Inflammation Array C1 by RayBiotech Inc. product image
Mouse Inflammation Array C1
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Antigen Information

Gene Symbols: CCL1 | CCL11 | CCL2 | CCL24 | CCL25 | CCL3 | CCL5 | CCL9 | CSF1 | CSF2 | CSF3 | CX3CL1 | CXCL1 | CXCL11 | CXCL12 | CXCL13 | CXCL5 | CXCL9 | FASLG | IFNG | IL10 | IL12A | IL13 | IL17A | IL1A | IL1B | IL2 | IL3 | IL4 | IL6 | IL9 | LEP | TIMP1 | TIMP2 | TNF | TNFRSF1A | TNFRSF1B | TNFSF8 | XCL1

Assay Format

Number of Targets Detected: 40

Species Detected: Mouse

Compatible Sample Types: Cell Culture Supernatants; Cell Lysates; Plasma; Serum; Tissue Lysates

Design Principle: Sandwich-based

Method of Detection: Chemiluminescence

Quantitative/Semi-Quantitative: Semi-Quantitative

Solid Support: Membrane

Product Specifications

Size: 4 or 8 Sample Kit

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Application Notes

Suggested Application

Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process

Kit Components

  • Mouse Inflammation Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Protocol Outline

  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.