The RayBiotech Monkeypox Virus 1-Step High Throughput PCR Kit is a ready to use PCR assay for the detection of MPXV DNA. It uses a fluorescent probe with specific primer sets to detect the J2R gene specific for the genome of MPXV. Primer and probe for an internal control, RNase P are also integrated in the assay to validate the assay quality. The kit also contains a proprietary buffer which enables the user to use crude skin swabs directly in their PCR reactions. By simply mixing the 1-Step Nucleic Acid Stabilization Buffer with the sample, the viral DNA is immediately released and stabilized, and interfering components of the human tissue are inactivated. This eliminates the need for laborious, costly DNA extraction and purification steps, drastically improving upon the traditional workflow of PCR, while removing potential sample loss and contamination.