Host DNA often negatively influences the sensitivity and specificity of molecular analysis of microorganisms in human and animal samples.
The proprietary MolYsis™ technology provides a solution for the removal of non-target host DNA and enrichment and isolation of bacterial and fungal DNA from intact microorganisms. All reagents and consumables provided with the kits are DNA-free ensuring pure DNA without reagent-borne contaminations. During the MolYsis™ process, human or animal cells are selectively lysed and the free DNA is degraded by a DNase treatment. Afterwards, a reagent called BugLysis is used for a broad-range lysis of Gram-positive and Gram-negative bacteria and fungi. The microbial DNA is purified with a column-based bind-wash-elute procedure providing pure, enriched microbial DNA.
Applications comprise next generation sequencing, amplicon cloning, real-time PCR, Sanger sequencing and DNA array analysis of microorganisms.
MolYsis™ Complete5 and MolYsis™ Complete10 are designed for medium (0.2-5ml) and large (5-10ml) sample volumes and are validated for a variety of human and animal body fluids.