Mass Spec-Compatible Yeast and Human Protein Extracts by Promega Corp.

Manufacturer Promega Corp.
The mass spectrometry-compatible yeast and human protein extracts are designed specifically for mass spectrometry applications. The extracts are predigested and cleaned up by solid-phase extraction for immediate use in liquid chromatography/mass spectrometry (LC/MS) analysis. Both the yeast and human extracts also are available in an intact undigested form to provide a test material for optimizing mass spec protein sample preparation. The yeast extracts are beneficial for users who prefer worki... Read more


Mass Spec-Compatible Yeast and Human Protein Extracts by Promega Corp. product image
Mass Spec-Compatible Yeast and Human Protein Extracts
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The mass spectrometry-compatible yeast and human protein extracts are designed specifically for mass spectrometry applications.

The extracts are predigested and cleaned up by solid-phase extraction for immediate use in liquid chromatography/mass spectrometry (LC/MS) analysis. Both the yeast and human extracts also are available in an intact undigested form to provide a test material for optimizing mass spec protein sample preparation. The yeast extracts are beneficial for users who prefer working with a relatively compact and well studied proteome, whereas the human extract provides opportunity for working with a complex proteome having a large dynamic range. Consistent extract protein composition is ensured by tight control over cell culture conditions and manufacturing process.

Lot-to-lot consistency of extracts is monitored by various protein and peptide qualitative and quantitation methods, including LC/MS and amino acid analysis (Table 1). Our manufacturing process assures compatibility with reverse phase liquid chromatography and mass spectrometry by monitoring nonspecific protein fragmentation, nonbiological post-translational modifications and, for digested extracts, minimal undigested peptides.


Features & Benefits:

  • Compatible with LC/MS instrumentation platforms.
  • Minimal nonbiological post-translational modifications.
  • Peptide quantity measured by AAA.
  • Model systems for method development/instrument monitoring.
  • Multiple formats (digest/intact).

Product Overview

Mass Spec-Compatible Yeast and Human Protein Extracts by Promega Corp. product image

Mass Spec-Compatible Yeast and Human Protein Extracts

Manufacturer Promega Corp.

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