Mass Spec-Compatible Yeast and Human Protein Extracts
The mass spectrometry-compatible yeast and human protein extracts are designed specifically for mass spectrometry applications. The extracts are predigested and cleaned up by solid-phase extraction for immediate use in liquid chromatography/mass spectrometry (LC/MS) analysis. Both the yeast and human extracts also are available in an intact undigested form to provide a test material for optimizing mass spec protei…
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Quality control for liquid chromatography/mass spectrometry.
I'm very pleased with the service that the Sales representative Paul Banks provides. When I ask for the quote he is very efficient and he always offers a good discount. The product is a high quality, very consistent between the batches, fits for purpose. I am very happy with the product.
Review Date: 27 Jun 2019 | Promega Corp.
The mass spectrometry-compatible yeast and human protein extracts are designed specifically for mass spectrometry applications.
The extracts are predigested and cleaned up by solid-phase extraction for immediate use in liquid chromatography/mass spectrometry (LC/MS) analysis. Both the yeast and human extracts also are available in an intact undigested form to provide a test material for optimizing mass spec protein sample preparation. The yeast extracts are beneficial for users who prefer working with a relatively compact and well studied proteome, whereas the human extract provides opportunity for working with a complex proteome having a large dynamic range. Consistent extract protein composition is ensured by tight control over cell culture conditions and manufacturing process.
Lot-to-lot consistency of extracts is monitored by various protein and peptide qualitative and quantitation methods, including LC/MS and amino acid analysis (Table 1). Our manufacturing process assures compatibility with reverse phase liquid chromatography and mass spectrometry by monitoring nonspecific protein fragmentation, nonbiological post-translational modifications and, for digested extracts, minimal undigested peptides.
Features & Benefits:
- Compatible with LC/MS instrumentation platforms.
- Minimal nonbiological post-translational modifications.
- Peptide quantity measured by AAA.
- Model systems for method development/instrument monitoring.
- Multiple formats (digest/intact).
Reference Yeast and Human Whole Cell Protein Extracts for Mass Spectrometry Instrument Performance Monitoring and Method Development
This application note, two protein extraction formats are described: pre-digested extracts, for monitoring instrument performance, and intact protein extracts, for optimization of sample preparation. To be compatible with mass spectrometry applications, extracts need to meet certain requirements, like freedom from detergents, high protein integrity and high reproducibility. Here, whole cell protein extracts from yeast and human cells that meet those criteria are described.
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