Member since: 2017
Organization: VIB/KU Leuven
Small bench top FACS that is robust and easy to use - highly recommended!
Application Area: Flow Cytometry
"For the MACsQuant or the Vyb as we call it, is a solid work horse of the lab. It goes from 9am to 4pm non-stop most days. Our use is mainly characterization of hematopoietic cells and the occassional adherent cell line. For 3 colour analysis its perfect. We have the three laser version and there is no real need for compensation if you use the vioBlue, FITC (GFP) and then the Y4 (PE/APC-Vio770 conjugated antibodies) channels. Miltenyi has a great selection of antibodies as well. We also do four colours very simply when we have mCherry which is detected in the Y2 channel. It can also do high throughput and we routinely run 96 well plates. Note - if you do not have the optional refilling station the running buffer will run out about 3/4 of the way through so you need to be there to replace it. The FACS also has a mix option as well so there is no problem with the cells settling in the bottom of the well either. Service: we have had no serious issues with the machine. Only once did we have serious blockage and at one stage one of the valves started to leak. But all were solved in a timely manner. They do come and update the software now and again but this also means you lose your data so you need to back this up. Analysis: we do all this in FlowJo. You do need to do Miltenyi Transform to see the cells but otherwise that's the only tweak required. Do note that the resolution of populations is not as good as a high end BD machine. But I guess this is why this machine is essentially 1/4 the price?! One nice feature is that it gives you a cell concentration as well because it takes a certain volume for each sample rather than continuously aspirate until it gets the number of stated events. All in all my impression from using it the last few years is that it is a great machine for all our routine 3-4 colour work. "
Member since: 2014
Organization: Michigan Tech University
Fast, rapid, fully automated multi-color flow cytometry
Application Area:Identity and characterization of all cells types dendritic cells, endothelial, T cells, tumor cells
"There are three lasers (405, 488, 561 nm), two scatter (FSC, SSC) and eight fluorescent channels, this allows for good flow cytometry results as compared to other 2 laser systems. It is fully automatic and there are cool color codes for each reagent which light up like mini disco lights. It is fast, cell counts are available in less than a minute,"
Member since: 2014
Organization: Universidad de Extremadura
Application Area:Cell Analysis, protein expression, fluorescent proteins screening
"We bought the instrument for screening samples for expression of fusion proteins with fluorescent proteins. Its clever laser design allows detection of 4 different fluorescent proteins at the same time, reducing time analysis and increasing throughput. FCS files can be read in any flow cytometry analysis software. But currently we are using it for a lot of different applications, from cell cycle analysis from fish or plants to blood immunophenotyping."
Member since: 2015
Organization: University of Gent
Application Area:Cell phenotyping, reporter gene expression analyses
"The machine is fine. We had some issues with the one we bought, several components had to be changed, eventually it got much more stable, the service engineers also got way more experience. Original software did not allow for much flexibility, but a new version came out and presumably some issues got fixed. Steep learning curve, but once you get some experience the setup is quite straightforward. 96 well plate sampler is a handy feature. Because of our experiments specifics, we need a flow cytometer in a flow cabinet. Macsquant is compact enough to fit in one."
Member since: 2013
Organization: Charité - Universtätsmedizin Berlin
Application Area:Flow Cytometry
"It is easy to use for automated sampling with a 96-well format. Automated staining of samples is possible. The after sale care is very good. However the software performance could be enhanced. An update to flowjo version 10 is a good step for the future improvements. It is very good value for money."
Member since: 2011
Organization: Ben-Gurion University of the Negev, Department of Clinical Biochemistry,
"The auto compensation option missing few basic things that Diva have."
On top of the performance, automation, compactness and convenience associated with the MACSQuant® Family of flow cytometers, the MACSQuant VYB comes with a uniquely configured optical bench, featuring violet, yellow, and blue lasers. It is a perfect match for labs utilizing fluorescent protein reporters or for labs that want to simply use PE and FITC conjugates.
Finding the right fit for fluorescent proteins with the yellow laser
Simplified data analysis tools
Express Modes are unique add-on features for the MACSQuantify™ Software. They are standardized data analysis tools that are optimized to automate flow cytometric measurements and analyses via predefined experiment settings, acquisition, and automated gating. Derived from mathematical algorithms, they reduce human error and therefore increase experimental reproducibility.