LOBSTR E. coli Expression Strain for One-step Purification by Kerafast

Manufacturer Kerafast  |   Model: EC1001& EC1002  |  Available Worldwide
Ideal for low-expressing protein targets, the LOBSTR E. coli expression strain allows for one-step polyhistidine-tag affinity purification by eliminating the two major E. coli contaminants. Traditionally, a major drawback to using polyhistidine-tag affinity purification for proteins expressed in E. coli is contamination resulting from the presence of two naturally histidine-rich proteins in E. coli, ArnA and SlyD. LOBSTR (low background strain) is an E. coli strain for the expression of recom... Read more


LOBSTR E. coli Expression Strain for One-step Purification by Kerafast product image
LOBSTR E. coli Expression Strain for One-step Purification

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Ideal for low-expressing protein targets, the LOBSTR E. coli expression strain allows for one-step polyhistidine-tag affinity purification by eliminating the two major E. coli contaminants.

Traditionally, a major drawback to using polyhistidine-tag affinity purification for proteins expressed in E. coli is contamination resulting from the presence of two naturally histidine-rich proteins in E. coli, ArnA and SlyD. LOBSTR (low background strain) is an E. coli strain for the expression of recombinant polyhistidine-tagged proteins that addresses this challenge.

LOBSTR maintains normal cell growth, but significantly reduces the polyhistidine-tag binding affinities of ArnA and SlyD. The strain therefore reduces contamination by those two proteins, yielding recombinant polyhistidine-tagged protein of higher purity. This allows for one-step purification of low-expressing recombinant proteins.

LOBSTR is based on BL21(DE3) and can be used like other commercially available competent cells. A BL21(DE3)-RIL version is also available, which contains extra copies of the argU, ileY, and leuW tRNA genes, as well as a chloramphenicol marker.

 

Features:

  • Yields recombinant polyhistidine-tagged protein of higher purity
  • Allows for one-step purification
  • Ideal for purifying challenging low-expressing protein targets

Applications:

  • Protein expression
  • Expression of 6xHistidine-tagged proteins
  • Polyhistidine-tag affinity purification