Member since: 2019
Organization: U Lisboa
Great results - can't live without this kit
Application Area: ELIZA reaction
"The Kinetic-QCL™ Bulk Kinetic Chromogenic LAL Assay for the detection of endotoxins is possibly the most stable kit ELISA kit you will ever find. It is highly robust - it will work in the worse conditions and still provide the expected results. It is not the cheapest kit, but surely it is totally worth it."
This is a bulk configuration of the KQCLTM Kinetic Chromogenic LAL Endotoxin Detection Assay and is available as a made-to-order product. This bulk kit configuration contains endotoxin from E. coli Strain O55:B5. Testing of specific lots for making larger product reserves is possible. Please contact your local sales representative for further details. The Kinetic-QCLTM Kinetic Chromogenic LAL Assay is a quantitative, kinetic assay for the detection of Gram-negative bacterial endotoxin. A sample is mixed with the reconstituted LAL reagent in a 96-well plate and placed in an incubating plate reader that measures absorbance at 405 nm. The reaction is automatically monitored over time for the appearance of a yellow color.
In the presence of endotoxin, the lysate will begin to cleave the chromogenic substrate, causing the solution to become yellow. The time required for the change is inversely proportional to the amount of endotoxin present. The concentration of unknown samples can then be calculated from a standard curve. It is the chromogenic nature of the Kinetic-QCLTM Kinetic Chromogenic LAL Assay that makes it the most appropriate choice for testing small volume parenterals, vaccines, antibiotics and biologicals. The Kinetic-QCLTM Assay is less impacted by inhibitory products which may interfere with the clotting mechanism in turbidimetric and gel clot assays. With a sensitivity range of 0.005 EU/ml - 50.0 EU/ml, the Kinetic-QCLTM Kinetic Chromogenic LAL Assay is the most sensitive of the LAL assays.