ioMicroglia Human iPSC-derived microglia
ioMicroglia are human induced pluripotent stem cell (iPSC)-derived microglia, precision reprogrammed using opti-ox™ technology. Within 10 days post-revival, ioMicroglia are ready for experimentation, expressing (>90%) key markers, including TMEM119, P2RY12, and IBA1. ioMicroglia display key functional traits including phagocytosis and proinflammatory cytokine secretion and can be co-cultured with ioGlutamatergic Neurons™.
ioMicroglia are delivered cryopreserved
ioMicroglia display ramified morphology
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bit.bio’s opti-ox powered precision cellular reprogramming converts human pluripotent stem cells into highly defined and functional microglia within days. ioMicroglia provide a consisntet, rapid, functional, and co-culture compatible hiPSC-based model for neurodegenerative disease research and drug development.
Key features:
- Quick: rapidly maturing human microglia that are ready to use within 10 days post-revival.
- Functional: ioMicroglia display key phagocytic and cytokine secretion functions.
- Co-culture compatible: suitable for co-culture studies with neurons.
Applications:
neurodegenerative disease modeling and drug development, neuroinflammation research, co-culture studies, and transcriptome analysis.
Leveraging CRISPR-ready microglia for functional genomics studies
Microglia play a pivotal role in maintaining neural health, defending against pathogens, and maintaining the brain’s functional equilibrium. CRISPR-ready ioMicroglia from bit.bio have been engineered with precision reprogramming technology to constitutively express Cas9, enabling high knockout efficiency at protein level. Discover how CRISPR-ready ioMicroglia retains all the defining features of ioMicroglia, with no adverse effects on reprogramming potential, transcriptional profile, or functionality.
ioMicroglia in coculture with ioGlutamatergic Neurons
In this video, bit.bio demonstrates the coculture of ioMicroglia with ioGlutamatergic neurons. The aim is to showcase the interaction and communication between these two cell types in a controlled laboratory environment.
ioMicroglia phagocytosing pHrodo labelled Zymosan A BioParticles
In this video, bit.bio demonstrates the process of ioMicroglia phagocytosing pHrodo labelled Zymosan A BioParticles. The video highlights the use of pHrodo labelling, which allows real-time visualization of the phagocytic activity.
ioMicroglia 40-minute recording
In this video, bit.bio presents a 40-minute recording focused on ioMicroglia. The aim is to capture and observe the behavior and dynamics of these immune cells in the brain over an extended period of time.
Microglia precision reprogrammed from human iPSCs expand the toolkit for neurodegenerative disease modeling
Discover how a leading contract research organization is utilizing hiPSC-derived microglia to provide more clinically relevant assays
