The biocompatibility of medical devices needs to be assessed as laid down in the ISO 10993 guideline. This include the cytotoxicity testing of extracts prepared from the material on L-929 fibroblast cells.
The biocompatibility assay kit is built on assay ready L-929 cells which can be used instantly for the test without pre-cultivation. Briefly, the cells are thawed, seeded into the provided assay plates and challenged with the extracts in serial dilutions. Viability of the cells is quantified by the addition of XTT, a metabolic dye for a colorimetric read-out, which is included in the kit as well as appropriate references materials.