Human/Mouse MAPK Phosphorylation Array by RayBiotech Inc.

Manufacturer RayBiotech Inc.  |   Model: AAH-MAPK-1
RayBio® C-Series Human/Mouse MAPK Phosphorylation Antibody Array 1 Kit. Detects 17 phosphorylated proteins in human or mouse samples. Suitable for all liquid sample types but intended for use with cell and tissue lysates.


Human/Mouse MAPK Phosphorylation Array by RayBiotech Inc. product image
Human/Mouse MAPK Phosphorylation Array
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Antigen InformationGene Symbols:

AKT1 | CREB1 | GSK3A | GSK3B | HSPB1 | MAP2K1 | MAP2K3 | MAP2K6 | MAPK1 | MAPK14 | MAPK8 | MTOR | RPS6KA1 | RPS6KA3 | RPS6KA4 | RPS6KB1 | TP53

Assay FormatPathway:

Akt Signaling; HER/ErbB Signaling; IGF Signaling; JAK/STAT Signaling; MAPK Signaling; NFkB Signaling; PI3K-AKT Signaling; Wnt/beta-Catenin Signaling

Number of Targets Detected:

17

Species Detected:

Human; Mouse

Compatible Sample Types:

Cell Culture Supernatants; Cell Lysates; Plasma; Serum; Tissue Lysates

Design Principle:

Sandwich-based

Method of Detection:

Chemiluminescence

Quantitative/Semi-Quantitative:

Semi-Quantitative

Solid Support:

Membrane

Product SpecificationsSize:

2, 4, or 8 Sample Kit

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Application NotesSuggested Application

Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process

Kit Components

  • Human/Mouse MAPK Phosphorylation Antibody Array C1 Membranes
  • Blocking Buffer
  • Detection Antibody Cocktail
  • 1,000X HRP-Anti-Rabbit-IgG Concentrate
  • 20X Wash Buffer I Concentrate
  • 20X Wash Buffer II Concentrate
  • 2X Cell Lysis Buffer Concentrate
  • Detection Buffer C
  • Detection Buffer D
  • 8-Well Incubation Tray w/ Lid
  • Protease Inhibitor Cocktail
  • 100x Phosphatase Inhibitor Cocktail I
  • Phosphatase Inhibitor Cocktail II
  • Plastic Sheets
  • Array Map Template
  • Manual

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP??s ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Protocol Outline

  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated anti-IgG
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.