Human Inflammation Array Q1 by RayBiotech Inc.

Manufacturer RayBiotech Inc.  |   Model: QAH-INF-1
Quantibody® Human Inflammation Array 1 Kit. Detects 10 Human Inflammatory Factors. Suitable for all liquid sample types.

Human Inflammation Array Q1 by RayBiotech Inc. product image
Human Inflammation Array Q1
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Antigen Information

Gene Symbols: CCL2; CXCL8; IFNG; IL10; IL13; IL1A; IL1B; IL4; IL6; TNF

Assay Format

Pathway: JAK/STAT Signaling; MAPK Signaling; NFkB Signaling

Number of Targets Detected: 10

Species Detected: Human

Compatible Sample Types: Cell Culture Supernatants; Cell Lysates; Other Body Fluids; Plasma; Serum; Tissue Lysates

Design Principle: Sandwich-based

Method of Detection: Fluorescence Laser Scanner

Quantitative/Semi-Quantitative: Quantitative

Solid Support: Glass Slide

Product Specifications

Size: 1, 2, or 4 Slide Kit (16 sub-arrays per slide)

Product Features

  • More cost-effective than traditional ELISA
  • High specificity and system reproducibility
  • Suitable for diverse sample types
  • Low sample volume requirement: 50 µL or less
  • Get results same day (6-hour processing time)
  • Well-suited for high throughput assays
  • Q Analyzer software provides one-step computation

Application Notes

Suggested Application

Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range

Kit Components

  • Human Inflammation Array Q1 Slide(s)
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Lyophilized Standard Mix
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated Fluor
  • Slide Washer/Dryer
  • Adhesive Plastic Strips
  • Manual

Other Materials Required

  • Distilled or deionized water
  • Small plastic boxes or containers
  • Pipettors, pipette tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner

Protocol Outline

  1. Dry the glass slide
  2. Prepare Standards
  3. Block array surface
  4. Incubate with Samples and Standards
  5. Incubate with Biotinylated Detection Antibody Cocktail
  6. Incubate with Streptavidin-Conjugated Fluor
  7. Disassemble the glass slide
  8. Scan with a gene microarray laser scanner
  9. Perform densitometry and analysis


For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.