Antigen Information
Accession Number: P05231
Gene ID: 3569
Gene Symbols: IFNB2; IL6
Protein Name & Synonyms: Interleukin-6 (IL-6) (B-cell stimulatory factor 2) (BSF-2) (CTL differentiation factor) (CDF) (Hybridoma growth factor) (Interferon beta-2) (IFN-beta-2)
Target Species: Human
Assay Format
Detection Range: 3 pg/ml - 1000 pg/ml
Recommended Dilution (serum & plasma): 2 fold
Sensitivity: 3 pg/ml
Specificity:This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Number of Targets Detected: 1
Species Detected: Human
Compatible Sample Types: Cell Culture Supernatants; Cell Lysates; Plasma; Serum; Tissue Lysates
Design Principle: Sandwich-based
Method of Detection: Colorimetric
Quantitative/Semi-Quantitative: Quantitative
Solid Support: 96-well Microplate
Product Specifications
Size: 1, 2, or 5 x 96-Well Strip Microplate Kit
Product Features
Additional Notes
Spiking & Recovery Results
Sample Type | Average % Recovery | Range (%) |
---|---|---|
Serum | 92.63 | 83-103 |
Plasma | 93.74 | 84-104 |
Cell culture media | 95.65 | 83-105 |
Linearity Results
Serum | Plasma | Cell Culture Media | ||
---|---|---|---|---|
1:2 | Average % of expected Range (%) |
95 84-103 |
96 85-105 |
97 84-105 |
1:4 | Average % of expected Range (%) |
96 83-105 |
95 84-103 |
97 85-105 |
Application Notes
Kit Components
Other Materials Required
Protocol Outline
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.