Status:
ReviewerMember since: 2018
Organization: University of Pittsburgh
Specific and reproducible results!
Application Area: Analyze chemo/cytokines in conditioned medium
"Used to detect the expression of chemo/cytokines in the conditioned medium of cancer cells co-cultured with macrophages. I obtained specific results, no background and reproducible data. I strongly suggest to other colleagues this product."
Status:
Advanced ReviewerMember since: 2013
Organization: MSKCC
Easy to use, good value for the money
Application Area:analyze signaling
"The kit is easy to use, has high quality and results are reproducible. The data would be more sensitive if half of the reagents are used. Value for the money is high."
Status:
ReviewerMember since: 2017
Organization: University of Texas Medical Branch
The high throughput capability of the system is a plus.
Application Area:analyze cytokines in response to infection of human organoids.
"The product is highly effective and is quite versatile."
Status:
ReviewerMember since: 2017
Organization: Department of cardillogt
Results attractive
Application Area:Biomedical research
"Very effective and cost saving. Simple to use and well recognised by experts in this area"
Status:
ReviewerMember since: 2017
Organization: Augusta University
Good. I recommend it.
Application Area:breast cancer research
"Very easy to use. Low time consuming. Easy to customized."
Status:
ReviewerMember since: 2017
Organization: ucla
Good to use
Application Area:gene regulation
"Good to use and easy to use. One of my colleagues is writing the paper based on the data"
Status:
Advanced ReviewerMember since: 2013
Organization: MSKCC
Easy to use, works every time!
Application Area:Multiplex analysis of RTK and cytokines
"A very basic and simple multiplex kit to use. Just like running western blot, but the output is "Dots"."
Assay Format
Number of Targets Detected
80
Species Detected
Human
Compatible Sample Types
Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Design Principle
Sandwich-based
Method of Detection
Chemiluminescence
Quantitative/Semi-Quantitative
Semi-Quantitative
Solid Support
Membrane
Target Names
ENA-78 (CXCL5), GCSF, GM-CSF, GRO alpha/beta/gamma, GRO alpha (CXCL1), I-309 (TCA-3/CCL1), IL-1 alpha (IL-1 F1), IL-1 beta (IL-1 F2), IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8 (CXCL8), IL-10, IL-12 p40/p70, IL-13, IL-15, IFN-gamma, MCP-1 (CCL2), MCP-2 (CCL8), MCP-3 (MARC/CCL7), M-CSF, MDC (CCL22), MIG (CXCL9), MIP-1 beta (CCL4), MIP-1 delta (CCL15), RANTES (CCL5), SCF, SDF-1 alpha (CXCL12 alpha), TARC (CCL17), TGF beta 1, TNF alpha, TNF beta (TNFSF1B), EGF, IGF-1, Angiogenin, Oncostatin M, Thrombopoietin (TPO), VEGF-A, PDGF-BB, Leptin, BDNF, BLC (CXCL13), Ck beta 8-1 (CCL23), Eotaxin-1 (CCL11), Eotaxin-2 (MPIF-2/CCL24), Eotaxin-3 (CCL26), FGF-4, FGF-6, FGF-7 (KGF), FGF-9, Flt-3 Ligand, Fractalkine (CX3CL1), GCP-2 (CXCL6), GDNF, HGF, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IL-16, IP-10 (CXCL10), LIF, Light (TNFSF14), MCP-4 (CCL13), MIF, MIP-3 alpha (CCL20), NAP-2 (PPBP/CXCL7), NT-3, NT-4, Osteopontin (SPP1), Osteoprotegerin (TNFRSF11B), PARC (CCL18), PLGF, TGF beta 2, TGF beta 3, TIMP-1, TIMP-2
Product Features
Suggested Applications
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.