Human Cytokine Array C5 (2) by RayBiotech Inc.

Manufacturer RayBiotech Inc.  |   Model: AAH-CYT-5
4.5
/
5.0
  |  7 reviews
RayBio® C-Series Human Cytokine Antibody Array 5 Kit. Detects 80 Human Cytokines. Suitable for all liquid sample types.


Human Cytokine Array C5 (2) by RayBiotech Inc. product image
Human Cytokine Array C5 (2)

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Average Rating: 4.5
7 Scientists have reviewed this product

5 out of 5
Ease of use
4 out of 5
After sales service
4 out of 5
Value for money


  • Status:

    Reviewer
  • Member since: 2018

  • Organization: University of Pittsburgh



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Specific and reproducible results!
Rating: 5.0

  • Application Area: Analyze chemo/cytokines in conditioned medium

"Used to detect the expression of chemo/cytokines in the conditioned medium of cancer cells co-cultured with macrophages. I obtained specific results, no background and reproducible data. I strongly suggest to other colleagues this product."

Review date: 12 Sep 2018 | Human Cytokine Array C5 (2)
  • Status:

    Advanced Reviewer

  • Member since: 2013

  • Organization: MSKCC



  • Ease of use
    4 out of 5
    After sales service
    3 out of 5
    Value for money
    4 out of 5
Easy to use, good value for the money
Rating: 3.7

  • Application Area:analyze signaling

"The kit is easy to use, has high quality and results are reproducible. The data would be more sensitive if half of the reagents are used. Value for the money is high."

Review date: 20 Jul 2017 | Human Cytokine Array C5 (2)
  • Status:

    Reviewer

  • Member since: 2017

  • Organization: University of Texas Medical Branch



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
The high throughput capability of the system is a plus.
Rating: 4.0

  • Application Area:analyze cytokines in response to infection of human organoids.

"The product is highly effective and is quite versatile."

Review date: 20 Jul 2017 | Human Cytokine Array C5 (2)
  • Status:

    Reviewer

  • Member since: 2017

  • Organization: Department of cardillogt



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Results attractive
Rating: 5.0

  • Application Area:Biomedical research

"Very effective and cost saving. Simple to use and well recognised by experts in this area"

Review date: 20 Jul 2017 | Human Cytokine Array C5 (2)
  • Status:

    Reviewer

  • Member since: 2017

  • Organization: Augusta University



  • Ease of use
    5 out of 5
    After sales service
    3 out of 5
    Value for money
    3 out of 5
Good. I recommend it.
Rating: 3.7

  • Application Area:breast cancer research

"Very easy to use. Low time consuming. Easy to customized."

Review date: 20 Jul 2017 | Human Cytokine Array C5 (2)
  • Status:

    Reviewer

  • Member since: 2017

  • Organization: ucla



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Good to use
Rating: 5.0

  • Application Area:gene regulation

"Good to use and easy to use. One of my colleagues is writing the paper based on the data"

Review date: 19 Jul 2017 | Human Cytokine Array C5 (2)
  • Status:

    Advanced Reviewer

  • Member since: 2013

  • Organization: MSKCC



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Easy to use, works every time!
Rating: 5.0

  • Application Area:Multiplex analysis of RTK and cytokines

"A very basic and simple multiplex kit to use. Just like running western blot, but the output is "Dots"."

Review date: 12 Jul 2017 | Human Cytokine Array C5 (2)

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Species Detected

Human

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Human Cytokine Antibody Array C5 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom) X-ray Film and a suitable film processor or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results store the entire kit frozen at -20°C upon arrival. Stored frozen the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Number of Targets Detected

80

Product Overview

Human Cytokine Array C5 (2) by RayBiotech Inc. product image

Human Cytokine Array C5 (2)

Manufacturer RayBiotech Inc.

4.5 / 5.0 | 7 reviews