Human BDNF ELISA by RayBiotech Inc.

Manufacturer RayBiotech Inc.  |   Model: ELH-BDNF
4.5
/
5.0
  |  2 reviews
RayBio® Human BDNF ELISA Kit for cell culture supernatants, plasma, and serum samples.


Human BDNF ELISA by RayBiotech Inc. product image
Human BDNF ELISA
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Average Rating: 4.5
2 Scientists have reviewed this product

5 out of 5
Ease of use
5 out of 5
After sales service
4 out of 5
Value for money


  • Status:

    Reviewer
  • Member since: 2017

  • Organization: Gazi üniversity medical faculty



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    3 out of 5
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I like this kit :)
Rating: 4.3

  • Application Area: I measured Serum and humor aquose BDNF levels

"Easy to apply unwritten dilution for the Humor aquose Our serum bdnf results same other publication"

Review date: 21 Jul 2017 | Human BDNF ELISA
  • Status:

    Advanced Reviewer

  • Member since: 2016

  • Organization: national veterinary laboratories, Islamabad Pakistan



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
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Excellent
Rating: 4.7

  • Application Area:Diagnosis and research

"It is an excellent and useful product. The protocol is user friendly and produces very reliable results. The time period is also very logical."

Review date: 13 Jul 2017 | Human BDNF ELISA

Product Features

Sensitivity
80 pg/ml

Looking for higher sensitivity? Check out our new Ultrasensitive Biomarker Testing Service powered by SIMOA™ technology.

Detection Range
80 pg/ml - 16 ng/ml
Recommended Dilution (serum & plasma)
5 - 500 fold
Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Specificity

This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN- gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1delta, PARC, PDGF, RANTES, SCF, TARC, TGF- beta, TIMP-1, TIMP-2, TNF- alpha, TNF- beta, TPO, VEGF.
Species Detected
Human
Protein Name & Synonyms
Brain-derived neurotrophic factor (BDNF) (Abrineurin)
Accession Number
P23560
Gene Names
BDNF
Gene ID
627

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.