Human BDNF ELISA by RayBiotech Inc.
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Status:
Reviewer
Member since: 2017
Organization: Gazi üniversity medical faculty
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- Ease of use
- After sales service
- Value for money
I like this kit :)
Application Area: I measured Serum and humor aquose BDNF levels
"Easy to apply unwritten dilution for the Humor aquose Our serum bdnf results same other publication"
Review date: 21 Jul 2017 | Human BDNF ELISA
Status:
Advanced Reviewer Member since: 2016
Organization: national veterinary laboratories, Islamabad Pakistan
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- Ease of use
- After sales service
- Value for money
Excellent
Application Area:Diagnosis and research
"It is an excellent and useful product. The protocol is user friendly and produces very reliable results. The time period is also very logical."
Review date: 13 Jul 2017 | Human BDNF ELISA
Antigen Information
Accession Number: P23560
Gene ID: 627
Gene Symbols: BDNF
Protein Name & Synonyms: BDNF
Target Species: Human
Assay Format
Detection Range: 80 pg/ml - 16 ng/ml
Recommended Dilution (serum & plasma): 5 - 500 fold
Sensitivity: 80 pg/ml
Specificity: This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN- gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1delta, PARC, PDGF, RANTES, SCF, TARC, TGF- beta, TIMP-1, TIMP-2, TNF- alpha, TNF- beta, TPO, VEGF.
Number of Targets Detected: 1
Species Detected: Human
Compatible Sample Types: Cell Culture Supernatants; Plasma; Serum
Design Principle: Sandwich-based
Method of Detection: Colorimetric
Quantitative/Semi-Quantitative: Quantitative
Solid Support: 96-well Microplate
Product Specifications
Size: 1, 2, or 5 x 96-Well Strip Microplate Kit
Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Product Overview
