HoloMonitor® M4 by Phase Holographic Imaging
Status:
Reviewer
Member since: 2019
Organization: Lund University
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- Ease of use
- After sales service
- Value for money
Every lab should have one!
Application Area: Single cell analysis for cell proliferation, migration and bulk analysis
"The beauty is in the single-cell analysis The microscope creates amazing time-lapse movies, and it is very easy to set up for time-lapse imaging. High incubator humidity to reduce media evaporation does, however, require regular service to clean the internal optics of the incubator tolerant microscope. Population-level cell analysis is faster and more convenient, but the absolute beauty of the microscope is the single-cell tracking and analysis. Though it is a bit frustrating sometimes, to know that there is a huge amount of data and time-lapse movies of single-cell tracking on my computer, and not enough time to analyze it. Also, the price is amazingly low. Every lab should have one!"
Review date: 19 Sep 2019 | HoloMonitor® M4
Status:
Reviewer Member since: 2019
Organization: University of Utah
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- Ease of use
- After sales service
- Value for money
Excellent instrument with great customer support and varied applications.
Application Area:Analysis of cell movement and growth.
"There is a bit of a steep learning curve for figuring out how to use the instrument and analyze the data, but once mastered, the HoloMonitor M4 is extremely convenient and useful for cell counting, proliferation assays, cell motility and migration assays, analysis of cell morphology and monitoring of cell heterogeneity and plasticity. The manufacturer also provides incredible support and instruction for learning how to use the instrument. It has become a staple for my lab both for daily tissue culture use (cell counting, looking at cells) and more complex live-imaging experiments."
Review date: 15 Aug 2019 | HoloMonitor® M4
Status:
Reviewer Member since: 2019
Organization: The University of Edinburgh
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- Ease of use
- After sales service
- Value for money
The HoloMonitor is a fantastic tool when studying biophysical properties of cells.
Application Area:Regenerative medicine
"We are very happy with the HoloMonitor M4. It is very easy to use, produces consistent results since nearly 4 years and is a great way to gather a lot of quantitative biophysical metrics on adherent cells in a label-free and real-time way. The after sale service is great, and we had fast answers to our questions and access to online assistance."
Review date: 15 Aug 2019 | HoloMonitor® M4
Status:
Reviewer Member since: 2019
Organization: Malmö University
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- Ease of use
- After sales service
- Value for money
Great results in no time!
Application Area:Analyze cell viability and cell characterization with different treatments
"With DHC we can easily do time-lapse analyses with different cell types and with different treatments. Not only cell analysis but also migration studies and motility assays. Easy to set up, run and analyze."
Review date: 09 Aug 2019 | HoloMonitor® M4
Status:
Reviewer Member since: 2018
Organization: Malmö University
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- Ease of use
- After sales service
- Value for money
Every laboratory working with cell cultures should have a HoloMonitor.
Application Area:Cancer cell cultures and the measurement of viability, death and motility
"The HoloMonitor provides the expert methodology for label-free imaging of living cells. The measurements are non-invasive, meaning that no fluorescent probes or dyes are used, which otherwise could affect the behaviour of the cells. The generated data can easily be analyzed both quantitatively and qualitatively with the provided software. We use the HoloMonitor for studying the effect of drugs on cell morphology, viability, death and motility of cancer cells. With this innovative technique, we can monitor cells over time and get reproducible results that are presented as images, time-lapse movies or diagrams. We have gained a lot by using the incubator-safe Holomonitor in our lab. The sales representatives are always helpful and easy to reach when we have questions or challenges with the techniqe. The HoloMonitor is very useful for everyone working in the cell biology and biomedicine area."
Review date: 09 Aug 2019 | HoloMonitor® M4
Status:
Reviewer Member since: 2019
Organization: Lund University
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- Ease of use
- After sales service
- Value for money
A new world opens up when you can follow your cells life in real-time.
Application Area:Migration, proliferation
"It is very easy to set up an experiment. It could be time consuming to analyze the images taken in H-studio, but it is much more efficient in AppSuite once you know what you want to look for. With the HoloMonitor M4 your experiments are really performed in real-time."
Review date: 08 Aug 2019 | HoloMonitor® M4
One important feature of HoloMonitor is the ability to analyze entire cell populations and the individual cells within them. This is possible using quantitative phase imaging (QPI) that enables easy identification of each individual cell and generates data for analysis of more than 30 cell-morphological parameters. From sequences of time-lapses images, the system software, HoloMonitor App Suite, helps the user to automatically extract and analyze kinetic live cell population data based on individual cell data.
The HoloMonitor platform offers a powerful and unique range of cell biological applications, including kinetic cell count and proliferation assays, cell motility and migration with single cell tracking module, kinetic cell death and other drug responses, cell cycle analysis etc.
When preferred, individual cell data – such as cell count, cell morphology, cell velocity, and cell division rate – can all be measured from the same experimental time-lapse data, without requiring additional experiments. Subpopulations of cells behaving differently that the average population can be identified and analyzed separately.
- Visualizes and quantifies cellular events. HoloMonitor is designed to generate both visual elements such as sequences of images and videos, and quantitative data, putting numbers on what can be seen.
- Incubator compatible design. Cell cultures can be continuously monitored and analyzed under physiologically optimal conditions, i.e. in the cell incubator or hypoxia chamber.
- Cell friendly and label free. The low-intensity, single-wavelength laser generates no heat reducing the risk of photo-damage to an absolute minimum. All HoloMonitor applications are totally label-free; no staining or contrast agents are required. The risk for toxic effects and/or masking of the effect of various conditions and/or target drug is thus avoided and results obtained without compromising cell integrity.
- Ideal for long term kinetic cell population studies. Images can be recorded with high temporal resolution (maximum image rate: 1 image/s), ensuring that important cellular events are not overlooked.
Application Areas
Cell Growth - Assess your cell culture integrity and kinetic cell proliferation
- The cell growth applications offer quick and quantitative live cell count and culture QC (quality control), of both adherent and suspension cells.
- Cellular growth rate, i.e. proliferation, can easily be determined, and details on the morphology of individual cells assessed.
Cell Movement - Distinguish between random cell motility and directed cell migration by imaging live cell movement
- Kinetic motility data with high level of automation
- Single cell tracking of cells of interest
- Live cell motility speed and cell migration velocity measurements
- Detailed understanding of wound healing processes
Cell Cycle - Analyze cells through rounds of replication
- No perturbation to the natural state and function of the cell with label-free analysis
- Robust measurements of mitosis and cytokinesis based on reliable automated segmentation
- Cytometric data with an effortless relationship between images and quantitative data
Drug Responses- Analyze cytotoxic effects and understand the cell death process
- Interactive dose response curves, with outcome for each condition displayed at each selected time point
- Determine cell proliferation, both by cell counting and by confluence, for various cell treatments simultaneously
- Understand drug effects through quantitative assessments of cell morphology
HoloMonitor provides a combination of quantitative and visual imaging data collected over time. The data can be accompanied with supporting videos which visualize how cancer cells shrivel up and die after being treated with a cancer drug, for example.
In addition to the application areas above the HoloMonitor features are optimal for capturing rare or transient cellular events like phagocytosis, cell engulfment, and cell activation and differentiation.
Moreover, since HoloMonitor leaves the cells unharmed additional studies on the cells, or the supernatant, can be performed after imaging.
Product Overview
