Status:
ReviewerMember since: 2019
Organization: Lund University
Every lab should have one!
Application Area: Single cell analysis for cell proliferation, migration and bulk analysis
"The beauty is in the single-cell analysis The microscope creates amazing time-lapse movies, and it is very easy to set up for time-lapse imaging. High incubator humidity to reduce media evaporation does, however, require regular service to clean the internal optics of the incubator tolerant microscope. Population-level cell analysis is faster and more convenient, but the absolute beauty of the microscope is the single-cell tracking and analysis. Though it is a bit frustrating sometimes, to know that there is a huge amount of data and time-lapse movies of single-cell tracking on my computer, and not enough time to analyze it. Also, the price is amazingly low. Every lab should have one!"
Status:
ReviewerMember since: 2019
Organization: University of Utah
Excellent instrument with great customer support and varied applications.
Application Area:Analysis of cell movement and growth.
"There is a bit of a steep learning curve for figuring out how to use the instrument and analyze the data, but once mastered, the HoloMonitor M4 is extremely convenient and useful for cell counting, proliferation assays, cell motility and migration assays, analysis of cell morphology and monitoring of cell heterogeneity and plasticity. The manufacturer also provides incredible support and instruction for learning how to use the instrument. It has become a staple for my lab both for daily tissue culture use (cell counting, looking at cells) and more complex live-imaging experiments."
Status:
ReviewerMember since: 2019
Organization: The University of Edinburgh
The HoloMonitor is a fantastic tool when studying biophysical properties of cells.
Application Area:Regenerative medicine
"We are very happy with the HoloMonitor M4. It is very easy to use, produces consistent results (for nearly 4 years) and is a great way to gather a lot of quantitative biophysical metrics on adherent cells in a label-free and real-time way. The after sales service is great, and we had fast answers to our questions and access to online assistance."
Status:
ReviewerMember since: 2019
Organization: Malmö University
Great results in no time!
Application Area:Analyze cell viability and cell characterization with different treatments
"With DHC we can easily do time-lapse analyses with different cell types and with different treatments. Not only cell analysis but also migration studies and motility assays. Easy to set up, run and analyze."
Status:
ReviewerMember since: 2018
Organization: Malmö University
Every laboratory working with cell cultures should have a HoloMonitor.
Application Area:Cancer cell cultures and the measurement of viability, death and motility
"The HoloMonitor provides the expert methodology for label-free imaging of living cells. The measurements are non-invasive, meaning that no fluorescent probes or dyes are used, which otherwise could affect the behaviour of the cells. The generated data can easily be analyzed both quantitatively and qualitatively with the provided software. We use the HoloMonitor for studying the effect of drugs on cell morphology, viability, death and motility of cancer cells. With this innovative technique, we can monitor cells over time and get reproducible results that are presented as images, time-lapse movies or diagrams. We have gained a lot by using the incubator-safe Holomonitor in our lab. The sales representatives are always helpful and easy to reach when we have questions or challenges with the techniqe. The HoloMonitor is very useful for everyone working in the cell biology and biomedicine area."
Status:
ReviewerMember since: 2019
Organization: Lund University
A new world opens up when you can follow your cells life in real-time.
Application Area:Migration, proliferation
"It is very easy to set up an experiment. It could be time consuming to analyze the images taken in H-studio, but it is much more efficient in AppSuite once you know what you want to look for. With the HoloMonitor M4 your experiments are really performed in real-time."
One important feature of HoloMonitor is the ability to analyze entire cell populations and the individual cells within them. This is possible using quantitative phase imaging (QPI) that enables easy identification of each individual cell and generates data for analysis of more than 30 cell-morphological parameters. From sequences of time-lapses images, the system software, HoloMonitor App Suite, helps the user to automatically extract and analyze kinetic live cell population data based on individual cell data.
The HoloMonitor platform offers a powerful and unique range of cell biological applications, including kinetic cell count and proliferation assays, cell motility and migration with single cell tracking module, kinetic cell death and other drug responses, cell cycle analysis etc.
When preferred, individual cell data – such as cell count, cell morphology, cell velocity, and cell division rate – can all be measured from the same experimental time-lapse data, without requiring additional experiments. Subpopulations of cells behaving differently that the average population can be identified and analyzed separately.
Application Areas
Cell Growth - Assess your cell culture integrity and kinetic cell proliferation
Cell Movement - Distinguish between random cell motility and directed cell migration by imaging live cell movement
Cell Cycle - Analyze cells through rounds of replication
Drug Responses- Analyze cytotoxic effects and understand the cell death process
HoloMonitor provides a combination of quantitative and visual imaging data collected over time. The data can be accompanied with supporting videos which visualize how cancer cells shrivel up and die after being treated with a cancer drug, for example.
In addition to the application areas above the HoloMonitor features are optimal for capturing rare or transient cellular events like phagocytosis, cell engulfment, and cell activation and differentiation.
Moreover, since HoloMonitor leaves the cells unharmed additional studies on the cells, or the supernatant, can be performed after imaging.