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Glycan Array 100 by RayBiotech Inc.

Manufacturer RayBiotech Inc.  |   Model: GA-Glycan-100  |  Available Worldwide
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Detection of binding partners for 100 different glycans in purified proteins, serum, plasma, cell culture supernatants, cell/tissue lysates or other body fluids.


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Glycan Array 100
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Assay Format

Number of Targets Detected: 100

Compatible Sample Types: Cell Culture Supernatants; Cell Lysates; Plasma; Purified Proteins; Serum; Tissue Lysates

Design Principle: Label-based; Sandwich-based

Method of Detection: Fluorescence Laser Scanner

Quantitative/Semi-Quantitative: Semi-Quantitative

Solid Support: Glass Slide

Product Specifications

Size: 4, 8, or 16 Sample Kit

Application Data

Kit Components

  • Dialysis Vials
  • Labeling Reagent
  • Stop Solution
  • Glycan Array Glass Slide Assembly
  • Sample Diluent
  • 20X Wash Buffer I
  • 20X Wash Buffer II
  • Cy3 equivalent dye-conjugated Streptavidin
  • Slide Washer/Dryer
  • Adhesive device sealer
  • Labeling Buffer
  • Floating Dialysis Rack

Other Materials Required

  • Detection antibodies of interest (For sandwich-based method only)
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Distilled or de-ionized water
  • 1.5ml Polypropylene microcentrifuge tubes
  • Gene microarray scanner or similar laser fluorescence scanner
  • Small plastic or glass containers
  • KCl, NaCl, KH2PO4 and Na2HPO4 (For label-based method only)
  • Beaker, stir plate and stir bar

Protocol Outline

  1. Dry the glass slide
  2. Block array surfaces
  3. Incubate samples (samples need to be biotinylated for the label-based approach)
  4. For the the sandwich-based principle, incubate with a detection antibody cocktail
    For the label-based principle, incubate the labeled-streptavidin.
  5. Incubate with Cy3 Equivalent Dye-Streptavidin
  6. Disassemble the glass slide
  7. Scan with a gene microarray laser scanner
  8. Perform densitometry and analysis

Storage/Stability

Upon receipt, all components of the RayBiotech Glycan Array 100 kit should be stored at -20°C. After initial use, remaining reagents should be stored at 4°C to avoid repeated freeze-thaw cycles and may be stored for up to 3 months (Labeling Reagent, Item B, should be prepared fresh each time before use). Unused glass slides should be kept at -20°C and repeated freeze-thaw cycles should be avoided (slides may be stored for up to 6 months). The entire kit should be used within 6 months of purchase.