GeoMx Digital Spatial Profiler is a novel platform developed by NanoString. Digital Spatial Profiling is based on nCounter® barcoding technology and enables spatially resolved, digital readout of up to 96 proteins or RNA targets in a multiplexed assay. The assay relies upon antibody or RNA probes coupled to photocleavable oligonucleotide tags. After hybridization of probes to slide-mounted formalin fixed paraffin-embedded (FFPE) tissue sections, the oligonucleotide tags are released from discrete regions of the tissue via UV exposure. Released tags are quantitated in a standard nCounter assay, and counts are mapped back to tissue location, yielding a spatially-resolved digital profile of analyte abundance.
NanoString’s GeoMx® Digital Spatial Profiler (DSP) combines the best of spatial and molecular profiling technologies by generating a whole tissue image at single cell resolution and digital profiling data for 10’s-1,000’s of RNA or Protein analytes for up to 12 tissue slides per day. This unique combination of high-plex, high-throughput spatial profiling enables researchers to rapidly and quantitatively assess the biological implications of the heterogeneity within tissue samples.
Highly multiplexed protein or RNA detection: up to 96 proteins and over 1000 RNA targets
Minimum sample: protein or RNA analysis from a single FFPE section
Morphological context: whole slide 4 color imaging to guide profiling
Maintains sample integrity: samples can be reused
Digital quantitation: up to 6 logs (base 10) dynamic range
Tunable resolution: select from 600 micron regions of interest down to single-cell
High throughput: up to 20 sections per day