Member since: 2020
Organization: Universidad Mayor de San Andrés
It makes easy to work on conservation genetics, especially in a low-income country!
Application Area: Tissue DNA isolation
"Ease of use: It's very easy to use and to isolate high-quality DNA from animal tissue, blood, and hair. After-sales care: It only needs to be at environmental temperature (average temperature in La Paz-Bolivia is 19 °C). Value for money: A kit for 100 samples has a cost of 500$ in Bolivia. Although it is not a low cost, it is one of the most inexpensive kits to isolate DNA that's high quality."
The Easy-DNA™ Kit is a fast and easy way to isolate high-quality, high-molecular weight genomic DNA (gDNA) from a wide range of cells and tissue types. Sample sizes can range from a single hair follicle up to 1 gram of mammalian tissue. Other samples tested include mouse tails, plant leaves, yeast, and E. coli cells. These samples yield high-quality DNA with an average size between 100 kb and 200 kb, which is suitable for PCR, DNA hybridization, genomic DNA library construction, and other applications. Advantages of the Easy-DNA™ Kit include:
Simple workflow, quality results
Optimized protocols for a variety of cell and tissue types are included in the instruction manual supplied with the kit. Generally, small-scale gDNA preparations are performed in microfuge tubes, in which samples are mixed with Lysis Solution and incubated at 65°C for 10 minutes. After extraction with chloroform (not included in kit), precipitation, and reconstitution of the DNA pellet, the preparation is complete. The kit contains a protein degradation reagent (for use with high-protein–containing samples such as connective tissue), mussel glycogen for use as a co-precipitant for dilute DNA samples, and an RNase solution.