DNA Clean & Concentrator™-5 by Zymo Research

"This has been very convenient clean-up / concentrator I always use with RNA isolation kit. Especially when handling small quantities of input samples, it is so crucial to be able to concentrate for the downstream application (e.g. 1st strand cDNA prep)"

"This product is very straightforward and user friendly. There are only a couple of steps and from start-to-finish, you can do it in about 10 minutes if you have everything setup ready to go. We particularly like using it when heat/vacuum methods to concentrate DNA are not available."

"Zymo's Clean & Concentrator-5 kit allows me to efficiently and easily clean my PCR product after amplification in as little as 15 minutes (depending on the number of samples being processed). There is almost no sample lost in the column during elution. This is definitely a very reliable product to use on crucial samples."

"The Zymo Clean & Concentrator kit has been a huge help for preparing samples for WGS. In a few simple steps I can concentrate very dilute samples into a small aliquot that is ready to ship for sequencing. The kit also removes EDTA from my samples that would otherwise have interfered with the WGS process. The cost is a small price to pay for better quality in my sequence data!"

"It is easy to use, but will have low yield when DNA amount is low."

"It is easy to use, but will have low yield when DNA amount is low."

"Attempted to use this product in order to troubleshoot why our libraries were stuck in the supernatant after a bead clean up. With our standard DNA, the columns worked great, but our problematic DNA staying stuck in the supernatant even with the Zymo clenaing column. Certainly a mystery."

"Generally, this one is good. When you don't want to do beads purification, column purification is easier. This one is very reliable, and easy to use. Especially the column design, you don't need to worry about residual alcohol on the O-ring."

"Purified DNA in just minutes. Easy to work with."

"DNA Clean & Concentrator™-5 kit is really good in terms of yield. It is easy to use. Handling of material is very easy. I found it to be the best kit so far."

"Very easy to use (10-15min for 3 samples) and yields high quality DNA. Great for digestion clean ups and for further clean ups after gel recovery."

"The use of this kit definitely simplified the cloning protocol in our lab, allowing the preparation of the plasmid vector in three-steps. Skipping the electophoresis on agarose gel lets us save time and to achieve a vector concentration that is far higher than that obtained with the purification from the gel band."

"Easy to use."

"Quick, 2 minute recovery of ultra-pure DNA from PCR, enzymatic reactions, and other sources. "

"Very easy to use, high yield, and quick."

"The product is very effective and the instructions are easy to follow."

"Quick to get high-purity DNA. Compatible with subsequent sequencing, PCR experiments, etc. Highly suggested!"

"The product is very effective, it gives us a good concentration of DNA. The instructions are easy to follow, the supplies are organized and labeled properly."

"The DNA Clean and Concentrator kit conveniently brings all reagents together in one kit with easy to follow instructions that work together, and as a result yield consistent results with little chance for error."

"Zymo PCR purification kit is reliable and always gives me high quality DNA purifications. Our lab have been using them for years and have never had a problem."

"Super simple column purification kit that lets you elute DNA in as little as 6 microlitres. We use it to concentrate and clean DNA after gel extractions for downstream cloning or sequencing purposes. "

"Easy and quick to use. Zymo support is great for troubleshooting and optimization. Makes dirty DNA extraction from non-model organisms usable for high sensitivity down stream applications. "

"Zymo clean and concentrate has always been reliable and gives high quality DNA. Our lab has used this product for years and has never had any issues."

"Elute in smallest volume possible - excellent for downstream applications!"

"This product is of great value, and is simple to use in the classroom. Students are able to access the protocols online and the approach is straightforward."

"This kit is a great alternative to ethanol precipitation for DNA concentration and cleaning up PCR products so they can be quantified. The instructions make modifying the procedure for different types of DNA easy."

"Its efficiency is much better than the other products with the same function. I wanted to purify products for nick translation experiments that have labeled dUTP on the strands and the efficiency of other kits on these products are very low. I tried DNA Clean & Concentrator-5 which could purify my product perfectly."

"We use this to purify PCR products when not run on a gel. This is a great product and easy to use. Small volume elution (6 ul) is the best aspect of this product because you can concentrate DNA in dilute samples."

"The product is very easy to use and takes less than 10 minutes for one sample. My products always end up with a higher concentration and show up cleaner on a UV-Vis Spec. I've been using it for a year now and I highly recommend this product!"

"Works as advertized, use it on NGS library and this help clean and concentrate the library after gel size selection."

"This kit, like most Zymo Research kits, is very straightforward and easy to use. The protocol takes about a few minutes to go through depending on how many samples you have. It does what it is supposed to do: give me higher yields from my PCR products if need be, which is useful for what we do here at RealSeq Biosciences."

"Effective at increasing concentration and purity of DNA samples post extraction."

"Very easy to use and quick. Yields are pretty good even with the low recommended volume for elution."

"Love the Zymo cleanup columns. I used them for DNA and RNA. There are no extra bits of residual wash buffer on the side like other columns, and the cleanup procedure is fast and easy."

"This product works extremely well for purifying and concentrating DNA for Illumina libraries. My NanoDrop readings showed heavy protein contamination after CTAB DNA extraction from plant tissue and this kit cleared it right up in less than half an hour. The new A260/A280 ratio was exactly 1.8 after using the kit. I have not tried it for large fragment DNA, though. I would definitely recommend this kit if you have some contamination in samples for PCR or short read library prep."

"For a very long time, I was using QIAGEN, and to a lesser extend Invitrogen products for DNA purification. Recently, I switched over to Zymo Research DNA Clean & Concentrator - 5 kit. Not only did I find it cheaper, but also easier and less time consuming. It also gives very reliable & consistent results. The quality of the product is better than what I have been using until now. This product is also more suitable for my purpose in terms of how well I can adapt in terms of starting material and elution volume, as well as the relative ease and convenience when using the product. "

"Tried using this kit to concentrate genomic DNA isolated from FFPE tissue for genotyping downstream. Kit was easy to use and quick, however, a lot of DNA seemed to be lost during recovery and my DNA was not concentrated, so in the end, it was not a good product for my needs."

"Enzymatic clean-up is great if you have lots of samples, but when I only have a dozen or so, the "Clean & Concentrator" kit gets it done in around 15-20 minutes. Slightly more expensive than enzymatic clean up, but excellent value among the various column-based clean up kits on the market, and of at least comparable quality. The only things I would change: It needs much less of the binding buffer (1 bottle is more than enough) and needs a little more of the elution buffer (10-20% more would be really useful)."

"Tried every other kit on the market, only this one preserved enough DNA for complex library generation downstream."

"My gDNA fecal extractions were low concentration and pretty contaminated, instead of doing them again I used this cleanup kit which was fast, easy, and worked like a charm. All my samples had a good concentration for sequencing after using this kit!"

"Easy to use, allows you to concentrate samples without affecting the quality of the sample (differently to methods that use warming)."

"It's a great product. Easy to use and very useful because we need to concentrate PCR products for our validation of sensitivity in-vitro and it really works well. In addition, the kit conserves the quality of the DNA. Excellent balance between price and quality. I would choose it every time!"

"A very simple protocol and reliable results. We get the best yields from this kit."

"The DNA Clean & Concentrator-5 is a great product that removes the need to carry out lengthy precipitations for routine DNA clean up. It is easy to use and allows you to elute in very low volumes with high yield and good purity values. Would recommend to everyone."

"This is a very easy way to clean up DNA fragments from a variety of upstream applications (PCR, restriction digests, etc). We get good yield and clean products for downstream steps. We even used this once to purify a radiolabelled probe away from unincorporated nucleotides."

"Kit is easy to use. Process is quick and yields clean product for downstream applications."

"Zymopure series columns work very well. Elution volume can be kept at low amount. Our DNA prep methods require concentration steps for NGS library prep. Hopefully, this kit will be cheaper in Japan."

"Great product, easy to use and implement. DNA recovery rate is high. Perfect for concentrating your DNA sample for low volume input assays, as well as buffer replacement tool when processing samples eluted in unknown buffer, and your downstream assays require low EDTA or salt content."

"Easy to use, gets the job done. Cheapest kit that I could find among competitors. Purifies PCR products >100bp flawlessly (and quickly; <5mins). I recommend this product"

"Easy-to-use, provides reproducible results. Perfect for low amounts in combination with class CI columns."

"This product is easy to use, straight-forward and requires only a minimum amount of time. It always works - which is not true for many other clean-up systems! Elution in quite small volumes is really cool."

"I didn't need to contact after sales service so please ignore my rating for the service. This is a convenient and simple product to use. It is very expensive considering the simplicity of the product."

"The product is very easy to use and it is very affordable price."

"Very quick protocol. Gives fairly good results and clean DNA"

"Easy to use, quick and simple prep that yielded decent results."

"The kit was easy to use and produced good clean DNA samples that we could use for NGS sequencing. There is considerable loss of sample so the kit requires a decent starting concentration of DNA but we were able to clean-up samples that we could not clean with more traditional methods (phenol extraction/ethanol precipitation)."

"I bought it because of its great price. After use, I found it have the same quality with Qiagen similar product."

"I used this product to clean DNA extracted from FFPE tissue and highly contaminated with phenol according to the spectrophotometer profile (ultra low 260/230 ratio, and 260/280 close to 1). The kit allowed to removal of all the contaminant, getting perfect 260/230 and 260/280 without major loss of template according to quantification via Qubit. The protocol is easy, as usual with products from Zymo, and the different parts of the kit can be bought separately. The elution can be restricted to a very low volume, allowing concentration of the template. The only negative aspect, is the necessity to use an elution buffer with pH>7 such as tris which my NGS facility does not accept."

"Recovery efficiency is high. Ease of use and better value at price comparing to QIAGEN MinElute PCR Purification Kit. Helpful, friendly, and immediate online tech support, should you have any questions."

"All the Zymo products I've used, including the DNA Clean & Concentrator™-5, are phenomenal. I work with very dirty samples - marine microbialites - contaminated with salts, extra polymeric substances, sediments, proteins, and, after collection, the preservative RNAlater. Zymo DNA and RNA Clean and Concentrator is the only product like it that has successfully cleaned my samples of all the contaminants remaining after extraction, including the phenol that carries over from the extraction itself. Additionally, my samples are very low in nucleic acid yield, so the added benefit of having one product to clean and concentrate means that I lose less material in downstream steps. I can even do a DNase treatment in the RNA C&C-5 kit. I'm very happy with Zymo's products."

"This kit was very easy to use, and had a straight forward protocol. Post cleaning DNA showed significant improvement in amplification."

"Easy to use, quick and simple prep that yielded decent results."

"Product is easy to use and yields are good. The protocol is also fast."

"I've been working in laboratories for many years and have had the chance to use many different PCR cleanup kits. I was able to get a free sample of the Zymo DNA clean & concentrate kits to prepare my PCR products for sanger sequencing. They worked great, and were much less expensive to use than my previous kit. The DNA clean & concentrate kits are very easy to use. One added feature that I really appreciated is that you can buy all of the kit components separate so if you have extra buffers you can just order more columns to stretch your dollar even more. I highly recommend this product."

"Really easy to use, instructions are super clear, and is a fast method to clean DNA for sequencing. The price is super affordable."

"These clean and concentrate cartridges do just that. They take and DNA prep, remove everything else and put it out in a few micro liters. They are well priced, easy to use, and the quick protocol is just the same with every sample. We keep them stocked in the lab to grab anytime there a a prep to be cleaned, enzyme removed, buffer changed, or just reduced in volume."

"This product allows elution of purified DNA into small volume in a great quality. The purification process is very fast. Each component of the kit can be purchased separately, which is very convenient and environment friendly."

"This product design is much better than QIAGEN and can efficiently recovery small amount of DNA in less than 10ul, adaptable for downstream application. Fast process and high yield "