Direct-zol™ RNA Miniprep
The Direct-zol™ RNA Kits provide a streamlined method for the purification of up to 100 ug (per prep) of high-quality RNA directly from samples in TRI Reagent® or similar. Spin column procedure bypasses phase separation and precipitation procedures, for non-biased recovery of miRNA.
Direct-zol RNA Miniprep Kit
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Will continue using for routine RNA prep since it is quite fast and easy
Isolate RNA for qPCR/gene expression analysis
Quick and easy to use. However, some difficult tissues/cells require extra steps and I'm still not sure how good it works for those (like PBMCs)
Review Date: 8 Feb 2023 | Zymo Research
Ready to use
RNA purification
It is easy to use by bypassing chloroform phase separation steps and provides purer RNA with min DNA contamination and with min loss
Review Date: 24 Jan 2023 | Zymo Research
Excellent quality of results
RNA extraction
I had problems of isolating Ribosomal RNA from my RiboTag tissues. I have tried many other methods until I found DirectZol! This is a game-changing kit that I finally got high-quality RNA for downstreaming RNA-seq. I also highly recommended the kit to my labmate, she got excellent results as well.
Review Date: 15 Dec 2022 | Zymo Research
Great product
RNA isolation
We got high quality RNA from mouse tissue using this kit and used for RNAseq. The steps and protocol are super easy to replicate.
Review Date: 21 Nov 2022 | Zymo Research
Easy to use, reliable
RNA extraction for qPCR, RNAseq, and Nanostring
This product is very easy to use. The protocol is thorough and easy to follow. The only thing that is a hassle is dealing with trizol, which one would have to do regardless of the kit used. Also sometimes the filter sticks in the tube too well. But other than that this kit has been a breeze for someone who used to do phenol-chloroform extraction.
Review Date: 26 Jul 2022 | Zymo Research
Great results!
RNA Isolation
This kit is super easy to use. The RNA yield is NGS-ready. The price comes down to about $4 per RNA, which is cheap. The technical support is some of the best I've received, helpful and knowledgeable.
Review Date: 20 Jun 2022 | Zymo Research
great kit, can't live without it
RNA preparation
Product is simple to use and gives excellent, clean RNA every time. Good value for the money and any time I've had an issue, Zymo has been quite helpful.
Review Date: 20 Jun 2022 | Zymo Research
Consistent, Effective performance
Molecular Biology
These kits work great for RNA isolation and are easy to follow the directions and even come with a DNase step which makes for very clean RNA
Review Date: 20 Jun 2022 | Zymo Research
Reliable, repeatable, high quality & high yield
Analyze tissue gene expression
The kits are easy to use and easy to train others to use. The columns are far better designed than other column purification columns (to retain maximum yield of high quality of your sample). The kits are reliable, repeatable, and sample quality is very consistent.
Review Date: 9 Jun 2022 | Zymo Research
It is perfect for RNA-Seq analysis.
RNA-Seq
The product is easy to use, the time to obtain the final RNA was short and the quality of the total RNA was excellent.
Review Date: 20 May 2022 | Zymo Research
The Direct-zol™ RNA MiniPrep provides a streamlined method for the purification of up to 50 µg (per prep) of high-quality RNA directly from samples in TRI-Reagent® or similar.
Total RNA (including small and non-coding RNAs) is effectively isolated from a variety of sample sources (cells, tissues, biological liquids, etc.) using this product. The procedure is easy: simply apply a sample in TRI-Reagent® to spin column and then spin, wash, and elute the RNA. No phase separation, precipitation, or post-purification steps are necessary.
Small RNAs (≥17 nucleotides) are efficiently and consistently recovered using this kit. The result is broad range purification of small and large RNAs suitable for subsequent RNA-based methods including RT-PCR, transcription profiling, hybridization, etc.
Features:
- The entire procedure typically takes about 5-10 minutes
- Quick, spin column purification of high-quality total RNA directly from TRI-Reagent®, TRIzol®, or similar
- Bypasses phase separation and precipitation procedures
- DNase I included for DNA-free RNA
