Dianthus for challenging affinity screening by NanoTemper Technologies

Dianthus is a plate-based and microfluidics-free affinity screening platform. Measurements are performed in solution and are mass-independent — ideal for succeeding at demanding screening campaigns With two methods for measuring molecular interactions, Spectral Shift and TRIC, Dianthus delivers high-quality data and requires less assay development to give you results from real-life samples

Today, the most important drug targets are super challenging when it comes to affinity screening. Immobilization-dependent SPR struggles with applications involving PROTAC binary and ternary complexes and intrinsically disordered proteins. These are precisely the applications where Dianthus excels

Here’s what makes Dianthus the perfect choice for challenging affinity screening campaigns:

• High signal-to-noise ratio: Reliable affinity measurements
• Mass independence: Measure binding with small and large molecules
• Broad range of affinities: From picomolar to millimolar for detection of strong and weak binders
• In-depth understanding of sample quality: Insights into aggregation induced by ligand

Dianthus' 384-well format and gRPC framework means it can be seamlessly integrated into many automation solutions that include robotic liquid handling and plate feeders for hours of uninterrupted and unattended operation