Member since: 2018
Organization: Arrowhead Pharmaceuticals
Serves its purpose very well, but could use some improvements
Application Area: RNA isolation for gene expression analysis by qPCR
"The functions of the Precellys evolution are great for our purposes, which mostly involve homogenization of various tissues for the purposes in Trizol for subsequent RNA isolation and gene expression analysis. However, I have a few grievances. As many scientists use Trizol/Tri-reagent for the purposes mentioned above, I was very disappointed to find that the lid and sample racks of the Precellys are not Trizol-proof, meaning they've developed many pockmarks and streaks because no matter how well you have tightened the homogenization tubes, there is an occasional leak. Additionally, the 7mL and 15mL tubes that the Precellys uses are great for their instrument, however, there are very few commercially available racks to stably hold large numbers of tubes (>20). There are centrifuge adaptors for centrifugation of the 7mL tubes, however there is not a corresponding adaptor for the 15mL tubes. The 15mL tubes fit into a 50mL conical adaptor for a bucket rotor, however, getting them back out is a problem. The "solution" of dumping the Precellys tubes from the bucket rotor is counter-productive to the centrifugation just performed."
Cryolys® Evolution is the only patented integrated cooling unit preventing the increase of temperature during lysing. Based on dry ice sublimation, it enhances the efficiency of molecular extraction, resulting in premium quality analysis, maintaining the desired temperature between 0 and 10°C before and during homogenization.
Cryolys® Evolution key benefits:
About Precellys’ expertise: