Cryolys Evolution - Cooling System for Thermo-Sensitive Samples
Cryolys Evolution is a patented cooling system compatible with Precellys Evolution tissue homogenizer. It prevents thermo-sensitive samples from heat degradation during homogenization process.
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Serves its purpose very well, but could use some improvements
RNA isolation for gene expression analysis by qPCR
The functions of the Precellys evolution are great for our purposes, which mostly involve homogenization of various tissues for the purposes in Trizol for subsequent RNA isolation and gene expression analysis. However, I have a few grievances. As many scientists use Trizol/Tri-reagent for the purposes mentioned above, I was very disappointed to find that the lid and sample racks of the Precellys are not Trizol-proof, meaning they've developed many pockmarks and streaks because no matter how well you have tightened the homogenization tubes, there is an occasional leak. Additionally, the 7mL and 15mL tubes that the Precellys uses are great for their instrument, however, there are very few commercially available racks to stably hold large numbers of tubes (>20). There are centrifuge adaptors for centrifugation of the 7mL tubes, however there is not a corresponding adaptor for the 15mL tubes. The 15mL tubes fit into a 50mL conical adaptor for a bucket rotor, however, getting them back out is a problem. The "solution" of dumping the Precellys tubes from the bucket rotor is counter-productive to the centrifugation just performed.
Review Date: 18 Apr 2018 | Bertin Technologies
- Efficient cooling: before, during and after processing
- No need for compressed air or power supply
- Accurate temperature monitored by Precellys® Evolution homogenizer software
- Minimal bench space
Cryolys® Evolution is the only patented integrated cooling unit preventing the increase of temperature during lysing. Based on dry ice sublimation, it enhances the efficiency of molecular extraction, resulting in premium quality analysis, maintaining the desired temperature between 0 and 10°C before and during homogenization.
Cryolys® Evolution key benefits:
- Achieve higher yield, greater quality and increased functionality
- Protect from enzymatic activities
- Ensure native protein conformation conservation
About Precellys’ expertise:
- 10 years of sample preparation expertise
- 30 000+ worldwide end users
- 2500+ applications and publications available on the Precellys Application Center
- High scientific support in many application fields: cancer research, neuroscience, forensic, pharmacology, microbiology research…
- Vast distribution network in over 50 countries
Revolutionizing biomolecular extraction: Master the art of cryogenic grinding
The extraction of biomolecules plays a crucial role in facilitating a wide range of bioanalytical techniques. These techniques include advanced methodologies such as next-generation sequencing and liquid chromatography, which rely on the extraction of biomolecules. The extraction and preservation of biomolecules face significant challenges due to their delicate nature, particularly during mechanical homogenization, whereby heat generation emerges as the primary threat to molecular integrity. Nevertheless, leading technology providers are making remarkable progress in delivering high-quality biological samples, while minimizing degradation and breakdown to a significant extent.
In this expert guide discover a range of cutting-edge instruments specifically engineered to address extraction challenges. By exploring real-life case studies and videos showcasing cryogrinding techniques, you will understand how to best optimize sample preparation, gain insights into tissue homogenization, and learn more about precise temperature regulation to enable you to master the best practices in the field.
This eBook will also explain:
- How to optimize extraction yield
- How to preserve sample integrity
- How to achieve successful integration of automated and semi-automated liquid handling technologies
Best practices in protein sample preparation with Precellys Evolution
For thermo-sensitive molecules such as proteins, sample preparation is a critical step before proceeding to molecular downstream analysis. In this application note, Bertin Instruments highlights the key considerations in protein sample preparation.
Precipitation of Proteins and Lipid Extraction From Homogenized Rat Skin Tissue
In this study, protein precipitation and bioactive lipid extraction from rat skin tissues is reported. The combination Solid Phase Extraction and liquid chromatography-tandem mass spectrometry (LCMS/MS) approach was used to measure concentrations of lipid mediators in these tissues. Rat skin tissues were homogenized using CKMix50_7mL Precellys® Lysing Kit and Precellys® Evolution tissue homogenizer combined with Cryolys® cooling unit. In addition, temperatures of homogenates were measured to investigate if temperature of complete homogenate of rat skin tissues remained below 40°C.
Best Practices for Tough Sample Grinding in Molecular Biology Using Precellys
The biggest challenge when working with fibrous, elastic and tough samples is efficient sample processing. Furthermore, generation of heat causes degradation and denaturation. Here, Bertin Instruments describes the improvement of extraction yields of tough samples using the Precellys® evolution homogenizer, thanks to 3D-bead beating technology. When paired with the cryolys evolution cooling unit, this technology can also prevent degradation of heat-sensitive biological samples.
Best Practices in Proteins Sample Preparation
This best practices guide for protein sample preparation using the Precellys® Evolution presents two application notes and two specific protocols for protein extraction.
The Cryolys Evolution – A cooling system for thermo-sensitive samples
In this video, Bertin Technologies introduces Cryolys Evolution, a patented cooling system specially developed for the Precellys Evolution tissue homogenizer. Based on dry ice sublimation, it enhances the efficiency of molecular extraction, resulting in premium quality analysis, maintaining the desired temperature between 0 and 10°C before and during the tissue homogenization. It is the ideal solution when working on native state thermo-sensitive molecules such as active RNA, siRNA, metabolites, enzymes, and proteins.
Achieve sensitive sample preparation with the Cryolys Evolution cooling unit
In this video, discover the Cryolys® Evolution, a patented cooling system compatible with the Precellys Evolution tissue homogenizer. End-users may control both devices from a single interface, enabling them to benefit from greater performances in a minimal bench space. Cryolys Evolution also prevents thermo-sensitive molecules, such as RNA, siRNA, metabolites, enzymes or proteins, from heat degradation during homogenization process. These molecules are subject to more and more investigations, especially in the fields of cancer research and pharmacology. Cryolys Evolution’s patented technology allows to maintain a low temperature (0°C- 10°C) before, during and after the grinding process with Precellys Evolution.
Your Neuroscience Playlist: 9 Essential Video Interviews
Watch video highlights from the Neuroscience 2018 annual meeting in San Diego, from the latest research presented, to the new technologies innovating neurobiology research
5 Essential Methods to Help Improve Protein-Based Experiments
In this SelectScience special feature dedicated to protein biology, discover methods to improve your experiments
Bertin Instruments Offers Free Demo for Neuroscience Researchers
Visit booth #2017 during the Society for Neuroscience's Annual Meeting on November 3-7, 2018, to discover innovative solutions to advance your research
