Gene encoding for the Nucleocapsid (N) proteinAssay FormatSensitivity:
25 viral RNA copies / µLCompatible Sample Types:
Extracted RNA (e.g., from a throat swab)Method of Detection:
Qualitative (by eye) & Semi-Quantitative (with plate reader)Product SpecificationsSize:
25 or 100 tests (tubes)Number of Targets Detected:
6 regions specific to the SARS-CoV-2 N gene. The regions selected for amplification do not share homology to nucleocapsid genes from any other coronaviruses.Plate Reader Compatibility:
Absorbance at 570 nm and 650 nmRT-LAMP Introduction
This assay utilizes reverse transcription loop-mediated isothermal amplification (RT-LAMP), which amplifies SARS-CoV-2 RNA without the need for thermal cycling. This is accomplished using specialized primers that enable continuous amplification of nucleic acids at 60°C. RT-LAMP is faster than traditional RT-PCR with a colorimetric endpoint readout. Thus, detection of viral RNA can be performed by eye or with a plate reader capable of reading absorbance at 570 nm and 650 nm.
This kit uses six primer-probe sets specific to the SARS-CoV-2 gene encoding for the nucleocapsid protein. The regions selected for amplification do not share homology to nucleocapsid genes from any other coronaviruses.
Absence of viral RNA is represented by a purple color (570 nm)(see picture above). The presence of viral RNA is represented by a blue color (650 nm). An OD ratio of 650 nm / 570 more than 1.0 indicates that SARS-CoV-2 viral RNA is present.Kit Components
|Component||25 Tests||100 Tests|
|RayBio® COVID-19 Rapid Master Mix||375 µl||1.50 ml|
|SARS-CoV-2 Primer Mix||125 µl||500 µl|
|SARS-CoV-2 Synthetic Nucleocapsid RNA (1000 copies/µl)||10 µl||10 µl|
|Ultra-pure DNase- and RNase-free Water||1 ml||1 ml|
This kit also has an option to include PCR tubes. 25 tests = 4 strips of 8 PCR tubes per strip. 100 tests = 13 strips of 8 PCR tubes per strip.Features