Frozen in 2mM HCl
Human placenta. Source material tested for hepatitis B antigen and HIV-1 antibody.
Electrophoretically homogeneous (SDS-PAGE)
Shown to promote attachment and spreading of HT-1080 human fibrosarcoma cells
Found negative for bacteria, fungi, and mycoplasma
Preparation and Storage
Stable for at least three months at -20°C. Avoid multiple freeze-thaws.
Effective as a gel or thin coating at a recommended concentration of 0.2-2 µg/cm2 of growth surface depending on cell type