ARM-D Kit, b-Lactamase by Streck Inc.

Manufacturer Streck Inc.  |  Available Worldwide
4.9
/
5.0
  |  2 reviews
The Streck ARM-D Kit, ß-Lactamase is a multiplex real-time PCR kit for the rapid detection of antibiotic resistance genes in Gram-negative bacteria. This comprehensive kit detects multiple variants within the following antimicrobial resistance gene families: KPC, NDM, OXA-48, IMP, VIM, DHA, CMY-2, CTX-M-14 and CTX-M-15.


ARM-D Kit, b-Lactamase by Streck Inc. product image
ARM-D Kit, b-Lactamase
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Average Rating: 4.9
2 Scientists have reviewed this product

5 out of 5
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money


  • Status:

    Reviewer
  • Member since: 2020

  • Organization: Wyoming Public Health Laboratory



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Good kit for gains of efficiency in the laboratory.
Rating: 5.0

  • Application Area: Great multiplexed assay for CRE detection

"This kit has simplified our workflow in the laboratory. Previously we were using the CDC designed assay for CRE detection. However, that is a single plex assay that needed more hands-on time. The Streck kit was easy to complete the verification study and is currently in use. For the full fledged panel, this is a good choice."

Review date: 17 Jul 2020 | ARM-D Kit, b-Lactamase
  • Status:

    Reviewer

  • Member since: 2020

  • Organization: Illinois dept. of Public Health



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
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We trust this this test to provide us with reliable and consistent results.
Rating: 4.7

  • Application Area:Antibiotic resistance genes testing

"Our laboratory has been using the Streck ARM-D PCR kits for beta lactamase gene detection for over two years now. The test is quick, accurate, and easy to use. We can quickly teach our technologists how to run and interpret the test. The test works very well on many PCR platforms, but we prefer the ABI 7500 DX. The PCR itself only takes 35 minutes so starting from a bacterial culture, extracting the DNA, setting up the master mix, and getting a result only takes about 2.5 hours. The nice thing is that the PCR cycling parameters are similar for the other ARM-D tests (ampC, OXA, and mcr). We are therefore able to perform them all on one PCR plate if we need to save time."

Review date: 24 Jun 2020 | ARM-D Kit, b-Lactamase

The Streck ARM-D Kit, β-Lactamase is part of a family of comprehensive, multiplex real-time PCR kits that detect over 1,000 antimicrobial resistance (AMR) gene variants from 26 different gene target families. Streck ARM-D Kits provide a rapid detection strategy for monitoring β-lactam antibiotic resistance, improving AMR surveillance, aid in antibiotic stewardship programs and support infection control programs. The ARM-D β-Lactamase kit detects over 350 variants in 9 clinically relevant carbapenemases, extended-spectrum beta-lactamases (ESBLs) and plasmid-mediated ampC gene families: CMY-2, CTX-M-14, CTX-M-15, OXA-48, IMP, VIM, DHA, KPC and NDM.

Streck ARM-D kits have a high degree of sensitivity and specificity for accurate, reliable results. Every kit includes validated positive controls for each target gene to monitor analytical process as well as an endogenous internal control (16SrRNA gene) to reduce false negatives due to PCR inhibition, DNA degradation or poor extraction.

The ARM-D β-Lactamase kit is an open-platform multiplex kit and is compatible with most 4-channel real-time thermal cyclers. The kit allows for batch testing, increasing throughput for high volume testing and reducing cost per sample compared to cartridge-based methods. An amplification protocol and analysis guide are available for the following instruments: Applied Biosystems 7500 Fast and 7500 Fast Dx, QuantStudio 7Flex, Bio-Rad CFX96 Touch and QIAGEN Rotor-Gene Q.

Features

  • Detects 9 antimicrobial resistance gene targets and over 350 variants
  • Protocol can be completed in less than one hour
  • Compatible with most 4-channel real-time PCR thermal cyclers
  • Includes a premixed 2X master mix: DNA Polymerase, dNTPs, MgCl2 and reaction buffer
  • Includes positive controls for each gene target to monitor analytical process
  • Amplification protocol and analysis guides available

Benefits

  • Avoid false negatives; detect gene variants not covered by cartridge-based kits
  • Detect resistance mechanisms prior to obtaining susceptibility results
  • Batch testing reduces cost per sample compared to cartridge-based methods
  • Ensure accurate and reliable results with a high degree of sensitivity and specificity
  • Screen bacterial isolates for AMR genes to complement phenotypic testing
  • Improve surveillance, aid antibiotic stewardship programs and support infection control programs

For Research Use Only. Not for use in diagnostic procedures. A CE version of this kit is also available.