Member since: 2020
Organization: Illinois dept. of Public Health
We trust this this test to provide us with reliable and consistent results.
Application Area: Antibiotic resistance genes testing
"Our laboratory has been using the Streck ARM-D PCR kits for beta lactamase gene detection for over two years now. The test is quick, accurate, and easy to use. We can quickly teach our technologists how to run and interpret the test. The test works very well on many PCR platforms, but we prefer the ABI 7500 DX. The PCR itself only takes 35 minutes so starting from a bacterial culture, extracting the DNA, setting up the master mix, and getting a result only takes about 2.5 hours. The nice thing is that the PCR cycling parameters are similar for the other ARM-D tests (ampC, OXA, and mcr). We are therefore able to perform them all on one PCR plate if we need to save time."
The Streck ARM-D Kit, β-Lactamase is part of a family of comprehensive, multiplex real-time PCR kits that detect over 1,000 antimicrobial resistance (AMR) gene variants from 26 different gene target families. Streck ARM-D Kits provide a rapid detection strategy for monitoring β-lactam antibiotic resistance, improving AMR surveillance, aid in antibiotic stewardship programs and support infection control programs. The ARM-D β-Lactamase kit detects over 350 variants in 9 clinically relevant carbapenemases, extended-spectrum beta-lactamases (ESBLs) and plasmid-mediated ampC gene families: CMY-2, CTX-M-14, CTX-M-15, OXA-48, IMP, VIM, DHA, KPC and NDM.
Streck ARM-D kits have a high degree of sensitivity and specificity for accurate, reliable results. Every kit includes validated positive controls for each target gene to monitor analytical process as well as an endogenous internal control (16SrRNA gene) to reduce false negatives due to PCR inhibition, DNA degradation or poor extraction.
The ARM-D β-Lactamase kit is an open-platform multiplex kit and is compatible with most 4-channel real-time thermal cyclers. The kit allows for batch testing, increasing throughput for high volume testing and reducing cost per sample compared to cartridge-based methods. An amplification protocol and analysis guide are available for the following instruments: Applied Biosystems 7500 Fast and 7500 Fast Dx, QuantStudio 7Flex, Bio-Rad CFX96 Touch and QIAGEN Rotor-Gene Q.
For Research Use Only. Not for use in diagnostic procedures. A CE version of this kit is also available.