The BÜHLMANN ACE kinetic assay uses the synthetic ACE substrate, FAPGG.
The ACE present in the sample cleaves the FAPGG into an amino acid derivative and a dipeptide which results in a decrease in absorbance at 340nm. The change in absorbance over a given time period can then be used to calculate the Angiotensin Converting Enzyme activity in the sample. The assay is supplied complete with calibrator and QC materials and provides a linear assay up to 150U/L. The substrate reagent is supplied as a ready-to-use, stable liquid but calibrator and controls are provided in a lyophilised state and reconstituted are stable for up to 6 months when stored at 2-8C.