Products & ReviewLife Sciences

ZymoPURE™ II Plasmid Midiprep Kit

The fastest spin-column based procedure for purifying up to 300 µg of ultra-pure endotoxin-free transfection-grade plasmid DNA.

Zymo Research

The supplier does not provide quotations for this product through SelectScience. You can search for similar products in our  Product Directory.

Average Rating 4.7

|72Scientists have reviewed this product

Ease of Use
After Sales Service
Value for Money
Write your own review

Easy to use and highly efficient


Average Rating 5.0

Application Area:

Purify plasmid from large culture

I have used it several times for overnight cultures of 50mL. Each time I obtained high yields with little hassle with the kit. It is easy to use and worth the money.

Review Date: 7 Dec 2022 | Zymo Research

Excellent product, easy to tailor to in house use, <3 hours for 8 samples!


Average Rating 5.0

Application Area:

Plasmid purification for mutagenesis and transfection

Cell Source: Thermo TOP10 Cells Culture Volume: 100mL Process Time: 8 samples takes ~2-3 hours from before culture spin down to completion of elution The protocol says 50mL of culture but we have adapted it for use with 100mL. The column say that they can hold up to 1.2mg of DNA and this culture volume works for us. This protocol is very resilient to user error and is consistent enough that we have had new interns reaching competency very quickly on this protocol. We have been using this product for over 1 year and the failure rate (<100ug) is <5%.

Review Date: 6 Dec 2022 | Zymo Research

Easy to use & adjust to experiments


Average Rating 5.0

Application Area:

Bacmid DNA Purification

This Zymo kit is a breeze to use- the instructions are clear, visual, and detailed, and the color-coded reagents make it easy to identify at a glance what is what. The kit is also readily adapted to the specifics of the experiment. If increasing the DNA yield or removing endotoxins is important, there are optional steps to include in your procedure. Also, replacing individual kit components does not require you to buy a whole new kit- every component has a part number that allows you to buy it directly from the manufacturer's website. Would highly recommend this product!

Review Date: 23 Nov 2022 | Zymo Research

Super easy for my first time doing a midi prep


Average Rating 5.0

Application Area:

Using the midi prep to expand DNA

I can't say better things about this kit. it was simple, streamline and came quick. I had no problems and had great results every time! I will never use another product!

Review Date: 21 Jun 2022 | Zymo Research

Good results, great price


Average Rating 4.3

Application Area:

Plasmid purification for cell line transfection

This worked as well as Qiagen's kit does, and at a fraction of the cost. I'm not going back to Qiagen, I'll tell you that much!

Review Date: 20 May 2022 | Zymo Research

Easy use great results


Average Rating 5.0

Application Area:

Extract plasmid

Easy use, great results, protocol can be perfected

Review Date: 20 May 2022 | Zymo Research

This is the product we have been using for years.


Average Rating 5.0

Application Area:

Plasmid purification

Easy to use and high yields. Has the option to remove the endotoxin.

Review Date: 30 Mar 2022 | Zymo Research



Average Rating 5.0

Application Area:

Molecular biology

The Zymo midi/maxi kits are great! It would be even better if the syringe filters/columns could fit into 50 mL conicals.

Review Date: 25 Jan 2022 | Zymo Research

Great product.


Average Rating 5.0

Application Area:

Plasmid for transfection

The sales team is very good and the product is wonderful.

Review Date: 16 Dec 2021 | Zymo Research

Absolutely love this kit! Never using anything else


Average Rating 5.0

Application Area:

Plasmid Extraction

When I first started my Ph.D., I had to do a lot of plasmid extractions. I struggled for months using different kits. I convinced my PI to purchase the Zymo Midiprep kit and I have successfully extracted high-quality plasmids without fail. Now, I can finally move on with my research and not wonder if I will get a high yield of high-quality plasmid. One of my favorite things about this kit is that the buffers are different colors. This is especially helpful for the neutralization step which allowed me to be confident in moving on to the next step. Another thing that I love about this kit is that the elution volume is 200uL. Other kits elute 1mL from the column which means that you need to precipitate the DNA in order to get a high concentration. This was especially important for me since I regularly needed 1ug/uL of plasmid DNA. I love everything about this kit and it is very quick to use. One of the best things is that it's cheap and of VERY good quality! Now, my lab has transitioned to using Zymo for Miniprep, Midiprep, and Maxiprep! Thank you, Zymo!!!

Review Date: 16 Nov 2021 | Zymo Research

ZymoPURE ™ Plasmid Isolation kits provide the fastest and simplest method available to efficiently isolate transfection quality plasmid DNA from E. coli. The ZymoPURE ™ Plasmid Midiprep kit features a spin column-based method for the purification of up to 300 µg of high-quality plasmid DNA in less than 18 minutes. The eluted plasmid DNA is ready for immediate use, avoiding the need for subsequent precipitation steps.

ZymoPURE ™ technology uses a modified alkaline lysis method and features novel binding chemistry that yields highly concentrated plasmid DNA (up to 3 µg/µl) directly from a spin column. The wash regimen has been optimized to ensure the plasmid DNA is free of endotoxins, salt, protein, and RNA resulting in plasmid DNA suitable for sensitive applications including transfection, plasmid-mediated gene silencing, cloning, and sequencing.

As an added convenience, the ZymoPURE ™ Plasmid Midiprep contains colored buffers that permit error-free visualization and identification of complete bacterial cell lysis and neutralization. Syringe filters are included for rapid clearing of the lysate and the unique spin-column design allows the binding step to be performed using a vacuum or table top centrifuge.

*Patent Pending

Product Overview