Vivapure AdenoPACK 20 - Small-scale virus purification
Vivapure AdenoPACK 20 is the small scale kit in the AdenoPACK series, purifying up to 1 x 1012 viral particles from 20 ml cell culture. Especially when it comes to testing new constructs, parallel and fast purifications of different adenoviruses are essential. This kit allows the rapid, simple and affordable spin column based purification of 6 different samples in parallel and bridges a gap in the CsCl density gradient method…
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Vivapure AdenoPACK 20 is the small scale kit in the AdenoPACK series, purifying up to 1 x 1012 viral particles from 20 ml cell culture. Especially when it comes to testing new constructs, parallel and fast purifications of different adenoviruses are essential. This kit allows the rapid, simple and affordable spin column based purification of 6 different samples in parallel and bridges a gap in the CsCl density gradient method – for the first time adenovirus can efficiently be purified from less than 100 ml cell culture volume.
• Spin column based kit
• Easy and fast parallel purification of multiple samples
• Virus purification based on membrane adsorber technology
• High-speed process completed in about 1 hour
• Excellent viral access provides quantitative recoveries
• Able to process 6 x 20 ml cell culture
• Recovers 1 x1011-12 viral particles from 20 ml cell culture
Treatment of Vivaspin® Concentrators for Improved Recovery of Low-Concentrated Protein Samples
With appropriate device size and membrane cut-off selected, Vivaspin® typically yield recoveries for the concentrated sample > 90% when the starting sample contains over 0.1 mg/ml protein of interest. The intention of the following “passivation” procedure, presented in the application note, is to improve recovery of protein samples in the nano- to microgram concentration range by pre-treating the device (membrane & plastic). This “passivation” procedure is designed for Vivaspin® ultrafiltration concentrators.
