Sartobind IEX Membranes

Adeno-associated viruses (AAV) belong to the family Parvoviridae, subfamily Parvovirus, genus Dependovirus. They are used as gene delivery vectors for human gene therapy. The advantages of AAV as a vector include e.g. its lack of pathogenicity, high titer, ease of manipulation, and ability to infect non-dividing cells. This application note describes the purification of adeno-associated virus using affinity chromatography with Sartobind® Membrane Adsorbers.

In this application note, ion exchange chromatography and Sartobind® membrane adsorbers were used to separate protein and peptide mixtures. All samples were prefiltered with a Minisart filter having 0.2 μm or a 0.45 μm pore size.

The isolation of DNA fragments from agarose gels is a standard method frequently used in molecular biology. A variety of isolation methods have been developed, all with advantages and disadvantages. This procedure describes the use of Sartobind D membranes for electrophoretic isolation of DNA fragments by insertion of small pieces of the weak anion exchange membrane into the agarose gel. It has the advantage of being inexpensive, efficient and has no contamination of agarose in the purified fragment.

In this application note, experiments with Sartobind Membrane Adsorbers (MA) show how optimization of conditions for binding and elution can lead to very high recovery of target protein in minutes. The convenient ready-to-use syringe formats are directly scaleable to Sartobind SingleSep and MultiSep process-scale modules.

New modular membrane chromatography solution for large-scale applications

Expands GE’s start-to-finish offering for cost efficient manufacture of biopharmaceuticals and increases global access to SSB’s innovative purification technology

Redesigned Sartobind® membrane adsorber capsules offer higher binding capacities, reduced void volumes, less buffer consumption and lower operational costs