Q5® High-Fidelity 2X Master Mix
Q5® High-Fidelity DNA Polymerase sets a new standard for both fidelity and robust performance. With the highest fidelity amplification available (~280 times higher than Taq), Q5 DNA Polymerase results in ultra-low error rates. Q5 DNA Polymerase is composed of a novel polymerase that is fused to the processivity-enhancing Sso7d DNA binding domain, improving speed, fidelity and reliability of performance. Working with uracil-…
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The Q5 master mix: Cost-effective product for any PCR application
Reliable polymerase suitable for any PCR
Q5 is a robust polymerase giving reproducible results using different PCR methods. The polymerase batches stay active beyond expiration date and the suggested reaction volumes can be significantly reduced (10μL) to save reagents. Moreover, the mastermix reduces pitpetting steps, thus the variability between reactions while saving again material.
Review Date: 18 Nov 2022 | New England Biolabs Inc.
Great overall HiFi polymerase
Routine HiFi PCR
Very robust HiFi polymerase, we use it daily in all our workflows that involve PCR, using a variety of inputs ranging from crude lysates, to gDNA and plasmid DNA.
Review Date: 18 Nov 2022 | New England Biolabs Inc.
Great experience and working very well
DNA amplification of highly complex gene
Achieve my expectation of results in comparison with other polymerases
Review Date: 29 Mar 2022 | New England Biolabs Inc.
High fidelity and reliable DNA polymerase.
PCR, DNA polymerase
Q5 master mix is a DNA polymerase which is very accurate and much faster than Taq with proofreading ability.
Review Date: 17 Jun 2020 | New England Biolabs Inc.
Q5® High-Fidelity DNA Polymerase sets a new standard for both fidelity and robust performance. With the highest fidelity amplification available (~280 times higher than Taq), Q5 DNA Polymerase results in ultra-low error rates. Q5 DNA Polymerase is composed of a novel polymerase that is fused to the processivity-enhancing Sso7d DNA binding domain, improving speed, fidelity and reliability of performance.
Working with uracil-containing DNA templates or using dUTP? Learn about Q5U Hot Start High-Fidelity DNA Polymerase (NEB #M0515).
- Highest fidelity amplification (~280X higher than Taq)
- Ultra-low error rates
- Superior performance for a broad range of amplicons (from high AT to high GC)
- Hot start and master mix formats available
