Newton EMCCD Detector
Both the Andor NewtonEM EMCCD and Newton conventional CCD detector systems have been optimized for high performance spectroscopic applications. All Newton detector systems employ low noise electronics, cooling to -100°C, up to 95% peak Quantum Efficiency (QE), multi-MHz readout, USB 2.0 connectivity and versatile readout modes. The NewtonEM employs Andor's electron multiplying CCD technology in an exclusive sensor f…
The supplier does not provide quotations for this product through SelectScience. You can search for similar products in our Product Directory.
Both the Andor NewtonEM EMCCD and Newton conventional CCD detector systems have been optimized for high performance spectroscopic applications.
All Newton detector systems employ low noise electronics, cooling to -100°C, up to 95% peak Quantum Efficiency (QE), multi-MHz readout, USB 2.0 connectivity and versatile readout modes.
The NewtonEM employs Andor's electron multiplying CCD technology in an exclusive sensor format optimized for ultra-low light, level spectroscopy applications. The combination of the Newton's low noise electronics, high QE, fast readout, and the on-chip amplication (electron multiplication) makes this detector unbeatable for the most demanding ultra-low light level spectroscopy applications, including single photon light level spectra. Dual output amplifiers also allow the detector system to operate in both the electron-multiplication mode and the conventional low noise readout modes, making the detector even more versatile for a wider variety of applications.
The Newton series of conventional CCD detectors employ industry leading low noise CCD sensors such as the 2048 x 512 and the 1024 x 256 pixel formats optimized for spectroscopy along with the standard features of ultra low noise electronics, deep cooling, high QE, multi-MHz readout, and USB 2.0 connectivity. The Newton CCD detector also provides dual output amplifiers for user selection of high sensitivity or high capacity operating modes.
High performance, and extreme versatility make this the ideal CCD detector to use with Andor's line of Shamrock imaging spectrographs to form a very powerful spectroscopy measurement system for use in even the most demanding applications.
Why Illumination Uniformity Matters
Reliable and accurate quantitative comparisons of fluorescence intensities across an image are impossible when the field illumination profile is not uniform. Uneven illumination of the sample will cause the intensity of a feature in one region of the field of view to register a different magnitude than the intensity of a feature of equal fluorophore concentration in another region of the field of view. When trying to determine the level of expression of a fluorescently tagged protein within a population of cells with uneven illumination, cells in the center of the image will appear to be brighter than those at the edges of the image.
This problem can lead to incorrect interpretation and analysis of the image data, especially when comparisons are made between images captured in different spectral channels (ie: FRET, calcium ratio imaging, colocalization, etc.).
UV/VIS Spectroscopy of Membrane Proteins Encapsulated into Artificial Bilayer Lipid Membranes
An artificial bilayer lipid membrane system was employed, featuring the oriented encapsulation of membrane proteins in a functionally active form. Nickel-nitrilo-tri-acetic acid-functionalized silica nanoparticles of a diameter of around 25 nm were used to attach the proteins via a genetically engineered histidine-tag in a uniform orientation. Subsequently the proteins were reconstituted within a phospholipid bilayer, formed around the particles by in-situ dialysis to form so-called proteo-lipobeads (PLBs). With a final size of about 50 nm, the PLBs could be employed for UV/VIS spectroscopy studies, particularly of multi-redox center proteins, since effects of light scattering are negligible. Andor Technology imaging instruments were employed including the Shamrock SR-303i and Newton CCD and EMCCD Cameras.
EMCCDs for Spectroscopy
Learn about Electron Multiplying CCD technology principles for spectroscopy, with new applications including Photoluminescence, Raman, FRET, Single Molecule, Transient and Tunnelling spectroscopies.
