Live Cell Imaging with White Light Confocal Microscopy

The Newton CCD camera is ideally suited to high-performance spectroscopic applications. The high-resolution sensors deliver up to 95% QE. Combined with Andor Technology’s low noise electronics, it provides convenient multi-Megahertz readout and USB connectivity. The camera utilizes a 1024 x 255 array of 26μm² pixels with thermoelectric cooling down to -100°C resulting in negligible dark current. The system offers the best performance for a wide range of spectroscopic applications across VUV-NIR wavelengths.

Both the Andor NewtonEM EMCCD and Newton conventional CCD detector systems have been optimized for high performance spectroscopic applications.   All Newton detector systems employ low noise electronics, cooling to -100°C, up to 95% peak Quantum Efficiency (QE), multi-MHz readout, USB 2.0 connectivity and versatile readout modes. The NewtonEM employs Andor's electron multiplying CCD technology in an exclusive sensor format optimized for ultra-low light, level spectroscopy applications. The combination of the Newton's low noise electronics, high QE, fast readout, and the on-chip amplication (electron multiplication) makes this detector unbeatable for the most demanding ultra-low light level spectroscopy applications, including single photon light level spectra. Dual output amplifiers also allow the detector system to operate in both the electron-multiplication mode and the conventional low noise readout modes, making the detector even more versatile for a wider variety of applications. The Newton series of conventional CCD detectors employ industry leading low noise CCD sensors such as the 2048 x 512 and the 1024 x 256 pixel formats optimized for spectroscopy along with the standard features of ultra low noise electronics, deep cooling, high QE, multi-MHz readout, and USB 2.0 connectivity. The Newton CCD detector also provides dual output amplifiers for user selection of high sensitivity or high capacity operating modes. High performance, and extreme versatility make this the ideal CCD detector to use with Andor's line of Shamrock imaging spectrographs to form a very powerful spectroscopy measurement system for use in even the most demanding applications.  

Andor's flagship live cell imaging software, designed with flexibility and power in mind. iQ - image and quantify - occupies a central role in their Revolution product range and provides optimized control of Andor's award winning iXon EMCCD cameras and automation hardware for a range of Bioimaging applications. iQ integrates a range of additional hardware for multi-parameter and multi-modal imaging, including devices such as confocal scanners, light sources including andor's own laser combiner, filter wheels and x,y, & z motorized stage control. Automation of this hardware with image capture is tightly controlled for optimum capture rates, and to minimise unnecessary light exposure for prolonged live cell imaging.iQ can be used for a range of bioimaging applications such as 3D live cell imaging, TIRF, ion imaging, bioluminescence and FRET. This makes iQ ideal for many research fields including cancer research, developmental biology, neuroscience, embryology, stem cell, and plant biology; capturing images from small subcellular components such as vesicles up to whole samples such as zebrafish and drosophila embryos. Continuous development and improvement ensures that Andor iQ represents a powerful and flexible core for live-cell imaging systems.iQ Live Cell Imaging Software Features: Power and flexibility Multi-dimensional core Fast 4D imaging Z-series imaging On-line data charting